View source for 2cdb

[[Image:2cdb.gif|left|200px]]<br />
<applet load="2cdb" size="450" color="white" frame="true" align="right" spinBox="true" 
caption="2cdb, resolution 1.600&Aring;" />
'''SULFOLOBUS SOLFATARICUS GLUCOSE DEHYDROGENASE 1 IN COMPLEX WITH NADP AND GLUCOSE'''<br />

==Overview==
The hyperthermophilic archaeon Sulfolobus solfataricus grows optimally, above 80 degrees C and utilizes an unusual, promiscuous, non-phosphorylative Entner-Doudoroff pathway to metabolize both glucose, and galactose. The first enzyme in this pathway, glucose dehydrogenase, catalyzes the oxidation of glucose to gluconate, but has been shown to, have activity with a broad range of sugar substrates, including glucose, galactose, xylose, and L-arabinose, with a requirement for the glucose, stereo configuration at the C2 and C3 positions. Here we report the, crystal structure of the apo form of glucose dehydrogenase to a resolution, of 1.8 A and a complex with its required cofactor, NADP+, to a resolution, of 2.3 A. A T41A mutation was engineered to enable the trapping of, substrate in the crystal. Complexes of the enzyme with D-glucose and, D-xylose are presented to resolutions of 1.6 and 1.5 A, respectively, that, provide evidence of selectivity for the beta-anomeric, pyranose form of, the substrate, and indicate that this is the productive substrate form., The nature of the promiscuity of glucose dehydrogenase is also elucidated, and a physiological role for this enzyme in xylose metabolism is, suggested. Finally, the structure suggests that the mechanism of sugar, oxidation by this enzyme may be similar to that described for human, sorbitol dehydrogenase.

==About this Structure==
2CDB is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus] with BGC, ZN, EDO and NAP as [http://en.wikipedia.org/wiki/ligands ligands]. Structure known Active Site: AC1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2CDB OCA]. 

==Reference==
The structural basis of substrate promiscuity in glucose dehydrogenase from the hyperthermophilic archaeon Sulfolobus solfataricus., Milburn CC, Lamble HJ, Theodossis A, Bull SD, Hough DW, Danson MJ, Taylor GL, J Biol Chem. 2006 May 26;281(21):14796-804. Epub 2006 Mar 23. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16556607 16556607]
[[Category: Single protein]]
[[Category: Sulfolobus solfataricus]]
[[Category: Danson, M.J.]]
[[Category: Hough, D.W.]]
[[Category: Lamble, H.J.]]
[[Category: Milburn, C.C.]]
[[Category: Taylor, G.L.]]
[[Category: Theodossis, A.]]
[[Category: BGC]]
[[Category: EDO]]
[[Category: NAP]]
[[Category: ZN]]
[[Category: glucose dehydrogenase]]
[[Category: mdr family]]
[[Category: oxidoreductase]]
[[Category: reductase]]

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