View source for 4gex

==Structure of a stabilised ceSAS-6 dimer, second crystal form==
<StructureSection load='4gex' size='340' side='right' caption='[[4gex]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4gex]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GEX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GEX FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2y3v|2y3v]], [[2y3w|2y3w]], [[3pyi|3pyi]], [[3q0y|3q0y]], [[3q0x|3q0x]], [[4g79|4g79]], [[4geu|4geu]], [[4gfa|4gfa]], [[4gfc|4gfc]]</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">sas-6, Y45F10D.9 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6239 Caenorhabditis elegans])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4gex FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gex OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4gex RCSB], [http://www.ebi.ac.uk/pdbsum/4gex PDBsum]</span></td></tr>
</table>
== Function ==
[[http://www.uniprot.org/uniprot/SAS6_CAEEL SAS6_CAEEL]] Required for centrosome duplication. Participates in centriole assembly.<ref>PMID:15572125</ref> <ref>PMID:15665853</ref>  
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Centrioles are evolutionary conserved organelles that give rise to cilia and flagella as well as centrosomes. Centrioles display a characteristic ninefold symmetry imposed by the spindle assembly abnormal protein 6 (SAS-6) family. SAS-6 from Chlamydomonas reinhardtii and Danio rerio was shown to form ninefold symmetric, ring-shaped oligomers in vitro that were similar to the cartwheels observed in vivo during early steps of centriole assembly in most species. Here, we report crystallographic and EM analyses showing that, instead, Caenorhabotis elegans SAS-6 self-assembles into a spiral arrangement. Remarkably, we find that this spiral arrangement is also consistent with ninefold symmetry, suggesting that two distinct SAS-6 oligomerization architectures can direct the same output symmetry. Sequence analysis suggests that SAS-6 spirals are restricted to specific nematodes. This oligomeric arrangement may provide a structural basis for the presence of a central tube instead of a cartwheel during centriole assembly in these species.

Caenorhabditis elegans centriolar protein SAS-6 forms a spiral that is consistent with imparting a ninefold symmetry.,Hilbert M, Erat MC, Hachet V, Guichard P, Blank ID, Fluckiger I, Slater L, Lowe ED, Hatzopoulos GN, Steinmetz MO, Gonczy P, Vakonakis I Proc Natl Acad Sci U S A. 2013 Jul 9;110(28):11373-8. doi:, 10.1073/pnas.1302721110. Epub 2013 Jun 24. PMID:23798409<ref>PMID:23798409</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Caenorhabditis elegans]]
[[Category: Erat, M C]]
[[Category: Vakonakis, I]]
[[Category: Alpha-beta protein]]
[[Category: Beta-sandwich]]
[[Category: Central tube]]
[[Category: Centriolar]]
[[Category: Cytoplasmic]]
[[Category: Structural protein]]