View source for 5bwi

==Crystallographic structure of a bacterial heparanase==
<StructureSection load='5bwi' size='340' side='right' caption='[[5bwi]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5bwi]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BWI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5BWI FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5bwi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5bwi OCA], [http://pdbe.org/5bwi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5bwi RCSB], [http://www.ebi.ac.uk/pdbsum/5bwi PDBsum]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
We report the structural and functional characterization of a novel heparanase (BpHep) from the invasive pathogenic bacterium Burkholderia pseudomallei (Bp), showing approximately 24% sequence identity with human heparanase (hHep). Site-directed mutagenesis studies confirmed the active site resi-dues essential for activity, and we found that BpHep has specificity for heparan sulfate. Finally, we describe the first heparanase X-ray crystal structure, which provides new insight into both substrate recognition and inhibitor design.

Functional and structural characterization of a heparanase.,Bohlmann L, Tredwell GD, Yu X, Chang CW, Haselhorst T, Winger M, Dyason JC, Thomson RJ, Tiralongo J, Beacham IR, Blanchard H, von Itzstein M Nat Chem Biol. 2015 Dec;11(12):955-7. doi: 10.1038/nchembio.1956. Epub 2015 Nov, 2. PMID:26565989<ref>PMID:26565989</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5bwi" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Blanchard, H]]
[[Category: Yu, X]]
[[Category: Glucuronidase]]
[[Category: Hydrolase]]