View source for 1bdb

[[Image:1bdb.gif|left|200px]]<br />
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'''CIS-BIPHENYL-2,3-DIHYDRODIOL-2,3-DEHYDROGENASE FROM PSEUDOMONAS SP. LB400'''<br />

==Overview==
cis-Biphenyl-2,3-dihydrodiol-2,3-dehydrogenase (BphB) is involved in the, aerobic biodegradation of polychlorinated biphenyls (PCBs). The crystal, structure of the NAD+-enzyme complex was determined by molecular, replacement and refined to an R-value of 17.9% at 2.0 A. As a member of, the short-chain alcohol dehydrogenase/reductase (SDR) family, the overall, protein fold and positioning of the catalytic triad in BphB are very, similar to those observed in other SDR enzymes, although small differences, occur in the cofactor binding site. Modeling studies indicate that the, substrate is bound in a deep hydrophobic cleft close to the nicotinamide, moiety of the NAD+ cofactor. These studies further suggest that Asn143 is, a key determinant of substrate specificity. A two-step reaction mechanism, is proposed for cis-dihydrodiol dehydrogenases.

==About this Structure==
1BDB is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_sp. Pseudomonas sp.] with NAD as [http://en.wikipedia.org/wiki/ligand ligand]. Structure known Active Site: CAT. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1BDB OCA]. 

==Reference==
Crystal structure of cis-biphenyl-2,3-dihydrodiol-2,3-dehydrogenase from a PCB degrader at 2.0 A resolution., Hulsmeyer M, Hecht HJ, Niefind K, Hofer B, Eltis LD, Timmis KN, Schomburg D, Protein Sci. 1998 Jun;7(6):1286-93. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9655331 9655331]
[[Category: Pseudomonas sp.]]
[[Category: Single protein]]
[[Category: Hecht, H.J.]]
[[Category: Hofer, B.]]
[[Category: Huelsmeyer, M.]]
[[Category: Niefind, K.]]
[[Category: Schomburg, D.]]
[[Category: Timmis, K.N.]]
[[Category: NAD]]
[[Category: nad-dependent oxidoreductase]]
[[Category: pcb degradation]]
[[Category: short-chain alcohol dehydrogenase]]

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