View source for 2wbl


==THREE-DIMENSIONAL STRUCTURE OF A BINARY ROP-PRONE COMPLEX==
<StructureSection load='2wbl' size='340' side='right' caption='[[2wbl]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2wbl]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WBL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WBL FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wbl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wbl OCA], [http://pdbe.org/2wbl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2wbl RCSB], [http://www.ebi.ac.uk/pdbsum/2wbl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2wbl ProSAT]</span></td></tr>
</table>
== Function ==
[[http://www.uniprot.org/uniprot/ROGF8_ARATH ROGF8_ARATH]] Guanine-nucleotide exchange factor (GEF) that acts as an activator of Rop (Rho of plants) GTPases by promoting the exchange of GDP for GTP. Active as homodimer.<ref>PMID:17218277</ref>  [[http://www.uniprot.org/uniprot/RAC2_ARATH RAC2_ARATH]] Inactive GDP-bound Rho GTPases reside in the cytosol, are found in a complex with Rho GDP-dissociation inhibitors (Rho GDIs), and are released from the GDI protein in order to translocate to membranes upon activation (By similarity). 
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wb/2wbl_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2wbl ConSurf].
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== Publication Abstract from PubMed ==
Abstract Guanine nucleotide exchange factors (GEFs) catalyze the activation of GTP-binding proteins (G proteins) in a multi-step reaction comprising intermediary complexes with and without nucleotide. Rho proteins of plants (ROPs) are activated by novel RopGEFs with a catalytic PRONE domain. We have previously characterized structures of GDP-bound ROP and a ternary complex between PRONE and ROP including loosely bound GDP. Now, we complete the molecular snapshots of the RopGEF reaction with the nucleotide-free ROP-PRONE structure at 2.9 A. The binary complex surprisingly closely resembles the preceding ternary intermediate including an unusually intact P-loop in the G protein. A striking difference is the prominent contact of the invariant P-loop lysine to a conserved switch II glutamate in ROP, favoring a key role of this interaction in driving out the nucleotide. The nucleotide-free state is supported by additional interactions involving the essential WW-motif in PRONE. We propose that this GEF region stabilizes the intact P-loop conformation, which facilitates re-association with a new nucleotide and further promotes the overall exchange reaction. With our novel structure we provide further insights into the nucleotide exchange mechanism and present a first example of the complete GEF reaction at a molecular level.

3D structure of a binary ROP-PRONE complex: the final intermediate for a complete set of molecular snapshots of the RopGEF reaction.,Thomas C, Fricke I, Weyand M, Berken A Biol Chem. 2009 Mar 31. PMID:19335195<ref>PMID:19335195</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 2wbl" style="background-color:#fffaf0;"></div>

==See Also==
*[[GTP-binding protein|GTP-binding protein]]
*[[Rho guanine nucleotide exchange factor|Rho guanine nucleotide exchange factor]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Arath]]
[[Category: Berken, A]]
[[Category: Fricke, I]]
[[Category: Thomas, C]]
[[Category: Weyand, M]]
[[Category: Complex of prone-gef with rop substrate]]
[[Category: Gtp-binding]]
[[Category: Lipoprotein]]
[[Category: Membrane]]
[[Category: Methylation]]
[[Category: Nucleotide- binding]]
[[Category: Nucleotide-binding]]
[[Category: Prenylation]]
[[Category: Signaling protein]]