View source for 2fco

[[Image:2fco.gif|left|200px]]

 {{Structure
|PDB= 2fco |SIZE=350|CAPTION= <scene name='initialview01'>2fco</scene>, resolution 1.40&Aring;
|SITE= 
|LIGAND= <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>
|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Crossover_junction_endodeoxyribonuclease Crossover junction endodeoxyribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.22.4 3.1.22.4] </span>
|GENE= RecU/PrfA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1462 Geobacillus kaustophilus])
|DOMAIN=
|RELATEDENTRY=
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fco FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fco OCA], [http://www.ebi.ac.uk/pdbsum/2fco PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2fco RCSB]</span>
}}

'''Crystal Structure of Bacillus stearothermophilus PrfA-Holliday Junction Resolvase'''


==Overview==
Here we report a high resolution structure of RecU-Holliday junction resolvase from Bacillus stearothermophilus. The functional unit of RecU is a homodimer that contains a "mushroom" like structure with a rigid cap and two highly flexible loops extending outwards. These loops appear to be highly flexible/dynamic, and presumably are directly involved in DNA binding and holding it for catalysis. Structural modifications of both the protein and DNA upon their interaction are essential for catalysis. An Mg2+ ion is present in each of the two active sites in this homodimeric enzyme, and two water molecules are coordinated with each Mg2+ ion. Our data are consistent with one of these water molecules acting as a nucleophile and the other as a general acid. The identities of the general base and general acid involved in catalysis and the Lewis acid that stabilizes the pentacovalent transition state phosphate ion are proposed. A model for the RecU-Holliday junction DNA complex is also proposed and discussed in the context of DNA binding and cleavage.

==About this Structure==
2FCO is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Geobacillus_kaustophilus Geobacillus kaustophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FCO OCA]. 

==Reference==
Structure, flexibility, and mechanism of the Bacillus stearothermophilus RecU Holliday junction resolvase., Kelly SJ, Li J, Setlow P, Jedrzejas MJ, Proteins. 2007 Sep 1;68(4):961-71. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17557334 17557334]
[[Category: Crossover junction endodeoxyribonuclease]]
[[Category: Geobacillus kaustophilus]]
[[Category: Single protein]]
[[Category: Jedrzejas, M J.]]
[[Category: Li, J.]]
[[Category: crystal structure]]
[[Category: flexibility]]
[[Category: hydrolase]]

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