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==Crystal Structure of the Acyltransferase Domain of the Iterative Polyketide Synthase in Enediyne Biosynthesis Reveals the Molecular Basis of Substrate Specificity==
<StructureSection load='4amn' size='340' side='right'caption='[[4amn]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4amn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_53710 Atcc 53710]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AMN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4AMN FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4amm|4amm]], [[4amo|4amo]], [[4amp|4amp]]</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4amn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4amn OCA], [http://pdbe.org/4amn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4amn RCSB], [http://www.ebi.ac.uk/pdbsum/4amn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4amn ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Biosynthesis of the enediyne natural product dynemicin in Micromonospora chersina is initiated by DynE8, a highly reducing iterative type I PKS that assembles polyketide intermediates from the acetate units derived solely from malonyl-CoA. To understand the substrate specificity and the evolutionary relationship between the acyltransferase (AT) domains of DynE8, fatty acid synthase (FAS) and modular PKSs, we overexpressed a 44 kDa fragment of DynE8 (hereafter named ATDYN10) encompassing its entire AT domain and the adjacent linker domain. The crystal structure at 1.4 A resolution unveils a alpha/beta hydrolase and a ferredoxin-like subdomain with the Ser-His catalytic dyad located in the cleft between the two subdomains. The linker domain also adopts a alpha/beta fold abutting the AT catalytic domain. Co-crystallization with malonyl-CoA yielded a malonyl-enzyme covalent complex that most likely represents the acyl-enzyme intermediate. The structure explains the preference for malonyl-CoA with a conserved arginine orienting the carboxylate group of malonate and several non-polar residues that preclude alpha-alkyl malonyl-CoA binding. Co-crystallization with acetyl-CoA revealed two non-covalently bound acetates generated by the enzymatic hydrolysis of acetyl-CoA that acts as an inhibitor for DynE8. This suggests that the AT domain can upload the acyl groups from either malonyl-CoA or acetyl-CoA onto the catalytic Ser651 residue. However, while the malonyl group can be transferred to the acyl carrier protein (ACP) domain, transfer of the acetyl group to the ACP domain is suppressed. Local structural differences may account for the different stability of the acyl-enzyme intermediates.

Crystal structure of the acyltransferase domain of the iterative polyketide synthase in enediyne biosynthesis.,Liew CW, Nilsson M, Chen MW, Sun H, Cornvik T, Liang ZX, Lescar J J Biol Chem. 2012 May 15. PMID:22589546<ref>PMID:22589546</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4amn" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Atcc 53710]]
[[Category: Lescar, J]]
[[Category: Liew, C W]]
[[Category: Acyltransferase]]
[[Category: Dynemicin]]
[[Category: Polyketide synthase]]
[[Category: Transferase]]