View source for 8gpu


==YFV_E_YD6Fab_prefusion==
<StructureSection load='8gpu' size='340' side='right'caption='[[8gpu]], [[Resolution|resolution]] 2.79&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8gpu]] is a 18 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Yellow_fever_virus Yellow fever virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8GPU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8GPU FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8gpu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8gpu OCA], [https://pdbe.org/8gpu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8gpu RCSB], [https://www.ebi.ac.uk/pdbsum/8gpu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8gpu ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q89292_9FLAV Q89292_9FLAV] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The yellow fever virus (YFV) is a life-threatening human pathogen. Owing to the lack of available therapeutics, non-vaccinated individuals are at risk. Here, we isolated eight human monoclonal antibodies that neutralize YFV infection. Five recognized overlapping epitopes and exhibited potent neutralizing activity. Two (YD6 and YD73) were ultra-potent and conferred complete protection against the lethal challenge of YFV as both prophylactics and therapeutics in a mouse model. Crystal structures revealed that YD6 engaged the YFV envelope protein in both pre- and post-fusion states, suggesting viral inhibition by a "double-lock" mechanism. The recognition determinants for YD6 and YD73 are clustered at the premembrane (prM)-binding site. Notably, antibodies targeting this site were present in minute traces in YFV-infected individuals but contributed significantly to neutralization, suggesting a vulnerable supersite of YFV. We provide two promising candidates for immunotherapy against YFV, and the supersite represents an ideal target for epitope-based vaccine design.

A neutralizing-protective supersite of human monoclonal antibodies for yellow fever virus.,Li Y, Chen Z, Wu L, Dai L, Qi J, Chai Y, Li S, Wang Q, Tong Z, Ma S, Duan X, Ren S, Song R, Liang M, Liu W, Yan J, Gao GF Innovation (Camb). 2022 Sep 15;3(6):100323. doi: 10.1016/j.xinn.2022.100323., eCollection 2022 Nov 8. PMID:36199277<ref>PMID:36199277</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8gpu" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Yellow fever virus]]
[[Category: Gao GF]]
[[Category: Li Y]]
[[Category: Qi J]]
[[Category: Wu L]]
[[Category: Yan J]]