View source for 4rig


==Chimeric Glycosyltransferase LanGT2S8Ac==
<StructureSection load='4rig' size='340' side='right'caption='[[4rig]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4rig]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_cyanogenus Streptomyces cyanogenus] and [https://en.wikipedia.org/wiki/Streptomyces_fradiae Streptomyces fradiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RIG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4RIG FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4rig FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rig OCA], [https://pdbe.org/4rig PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4rig RCSB], [https://www.ebi.ac.uk/pdbsum/4rig PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4rig ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q9ZGC0_STRCY Q9ZGC0_STRCY] [https://www.uniprot.org/uniprot/Q9RPA7_STRFR Q9RPA7_STRFR] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The structures of the O-glycosyltransferase LanGT2 and the engineered, CC bond-forming variant LanGT2S8Ac show how the replacement of a single loop can change the functionality of the enzyme. Crystal structures of the enzymes in complex with a nonhydrolyzable nucleotide-sugar analogue revealed that there is a conformational transition to create the binding sites for the aglycon substrate. This induced-fit transition was explored by molecular docking experiments with various aglycon substrates.

Structural Characterization of O- and C-Glycosylating Variants of the Landomycin Glycosyltransferase LanGT2.,Tam HK, Harle J, Gerhardt S, Rohr J, Wang G, Thorson JS, Bigot A, Lutterbeck M, Seiche W, Breit B, Bechthold A, Einsle O Angew Chem Int Ed Engl. 2015 Jan 7. doi: 10.1002/anie.201409792. PMID:25581707<ref>PMID:25581707</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4rig" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Streptomyces cyanogenus]]
[[Category: Streptomyces fradiae]]
[[Category: Bechthold A]]
[[Category: Breit B]]
[[Category: Einsle O]]
[[Category: Gerhardt S]]
[[Category: Tam HK]]