View source for 2bit

[[Image:2bit.gif|left|200px]]<br />
<applet load="2bit" size="450" color="white" frame="true" align="right" spinBox="true" 
caption="2bit, resolution 1.71&Aring;" />
'''CRYSTAL STRUCTURE OF HUMAN CYCLOPHILIN D AT 1.7 A RESOLUTION'''<br />

==Overview==
In the pharmaceutical industry, knowledge of the three-dimensional, structure of a specific target facilitates the drug-discovery process., Despite possessing favoured analytical properties such as high purity and, monodispersion in light scattering, some proteins are not capable of, forming crystals suitable for X-ray analysis. Cyclophilin D, an isoform of, cyclophilin that is expressed in the mitochondria, was selected as a drug, target for the treatment of cardiac disorders. As the wild-type enzyme, defied all attempts at crystallization, protein engineering on the enzyme, surface was performed. The K133I mutant gave crystals that diffracted to, 1.7 A resolution using in-house X-ray facilities and were suitable for, soaking experiments. The crystals were very robust and diffraction was, maintained after soaking in 25% DMSO solution: excellent conditions for, the rapid analysis of complex structures including crystallographic, fragment screening.

==Disease==
Known disease associated with this structure: Hypertension, salt-sensitive essential, susceptibility to OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=605325 605325]]

==About this Structure==
2BIT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Active as [http://en.wikipedia.org/wiki/Peptidylprolyl_isomerase Peptidylprolyl isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.2.1.8 5.2.1.8] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BIT OCA]. 

==Reference==
Crystal engineering yields crystals of cyclophilin D diffracting to 1.7 A resolution., Schlatter D, Thoma R, Kung E, Stihle M, Muller F, Borroni E, Cesura A, Hennig M, Acta Crystallogr D Biol Crystallogr. 2005 May;61(Pt 5):513-9. Epub 2005, Apr 20. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15858260 15858260]
[[Category: Homo sapiens]]
[[Category: Peptidylprolyl isomerase]]
[[Category: Single protein]]
[[Category: Hennig, M.]]
[[Category: Schlatter, D.]]
[[Category: Stihle, M.]]
[[Category: Thoma, R.]]
[[Category: cis-tran-isomerization]]
[[Category: crystal engineering]]
[[Category: human]]
[[Category: isomerase]]
[[Category: mitochondrial protein]]

''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 21:03:09 2007''