View source for 2omi

[[Image:2omi.gif|left|200px]]<br />
<applet load="2omi" size="450" color="white" frame="true" align="right" spinBox="true" 
caption="2omi, resolution 2.24&Aring;" />
'''Structure of human insulin cocrystallized with protamine'''<br />

==Overview==
Insulin NPH (neutral protamine hagedorn) has for long been one of the most, important therapeutic formulations for the treatment of diabetes. The, protracted action profile of NPH formulations is gained from crystallizing, insulin with zinc in the presence of the basic poly-arginine peptide, protamine. In spite of its long history and successful use, the binding, mode of the insulin-protamine complex is not known. In this study, three, different systems were used to study protamine binding to insulin. In the, first system, crystals of an insulin-protamine complex grown in the, presence of urea and diffracting to 1.5A resolution were analyzed. In the, second system, a shorter peptide consisting of 12 arginine residues was, co-crystallized with insulin in order to reduce the flexibility and, thereby improve the electron density of the peptide. Both systems yielded, data to a significantly higher resolution than obtained previously. In, addition, a third system was analyzed where crystals of insulin and, protamine were grown in the absence of urea, with conditions closely, resembling the pharmaceutical formulation. Data from these NPH, microcrystals could for the first time be collected to 2.2A resolution at, a micro focused X-ray beamline. Analysis of all three crystal forms reveal, potential protamine density located close to the solvent channel leading, to the centrally located zinc atoms in the insulin hexamer and support, that protamine binds to insulin in a not well defined conformation.

==Disease==
Known diseases associated with this structure: Diabetes mellitus, rare form OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=176730 176730]], Hyperproinsulinemia, familial OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=176730 176730]], MODY, one form OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=176730 176730]]

==About this Structure==
2OMI is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with ZN, CL and RCO as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2OMI OCA]. 

==Reference==
Structural characterization of insulin NPH formulations., Norrman M, Hubalek F, Schluckebier G, Eur J Pharm Sci. 2007 Apr;30(5):414-23. Epub 2007 Jan 20. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17339105 17339105]
[[Category: Homo sapiens]]
[[Category: Protein complex]]
[[Category: Norrman, M.]]
[[Category: Schluckebier, G.]]
[[Category: CL]]
[[Category: RCO]]
[[Category: ZN]]
[[Category: insulin nph microcrystals]]

''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 23:13:07 2007''