View source for 2bhd

[[Image:2bhd.gif|left|200px]]<br />
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'''MG SUBSTITUTED E. COLI AMINOPEPTIDASE P IN COMPLEX WITH PRODUCT'''<br />

==Overview==
The effect of metal substitution on the activity and structure of the, aminopeptidase P (APPro) from Escherichia coli has been investigated., Measurements of activity in the presence of Mn2+, Mg2+, Zn2+, Na+, and, Ca2+ show that significant activity is seen only in the Mn-bound form of, the enzyme. The addition of Zn2+ to [MnMn(APPro)] is strongly inhibitory., Crystal structures of [MnMn(APPro)], [MgMg(APPro)], [ZnZn(APPro)], [ZnMg(APPro)], [Ca_(APPro)], [Na_(APPro)], and [apo(APPro)] were, determined. The structures of [Ca_(APPro)] and [Na_(APPro)] have a single, metal atom at their active site. Surprisingly, when a tripeptide substrate, (ValProLeu) was soaked into [Na_(APPro)] crystals in the presence of 200, mM Mg2+, the structure had substrate, but no metal, bound at the active, site. ... [[http://ispc.weizmann.ac.il/pmbin/getpm?16229471 (full description)]]

==About this Structure==
2BHD is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]] with MG and FLC as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.11.9 3.4.11.9]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BHD OCA]]. 

==Reference==
Structural and functional implications of metal ion selection in aminopeptidase P, a metalloprotease with a dinuclear metal center., Graham SC, Bond CS, Freeman HC, Guss JM, Biochemistry. 2005 Oct 25;44(42):13820-36. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16229471 16229471]
[[Category: Escherichia coli]]
[[Category: Protein complex]]
[[Category: Bond, C.S.]]
[[Category: Freeman, H.C.]]
[[Category: Graham, S.C.]]
[[Category: Guss, J.M.]]
[[Category: FLC]]
[[Category: MG]]
[[Category: aminopeptidase]]
[[Category: dinuclear hydrolase]]
[[Category: manganese]]
[[Category: metal-binding]]
[[Category: metalloenzyme]]
[[Category: metalloprotease]]
[[Category: pita-bread fold]]
[[Category: product complex]]
[[Category: proline-specific peptidase]]

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