View source for 1oad

[[Image:1oad.gif|left|200px]]<br />
<applet load="1oad" size="450" color="white" frame="true" align="right" spinBox="true" 
caption="1oad, resolution 1.5&Aring;" />
'''GLUCOSE ISOMERASE FROM STREPTOMYCES RUBIGINOSUS IN P21212 CRYSTAL FORM'''<br />

==Overview==
Glucose isomerase from Streptomyces rubiginosus was crystallized in two, forms: I222, with one molecule of 44 kDa in the asymmetric unit, and, P2(1)2(1)2, with two unique molecules. The I222 structure is known, but, the P2(1)2(1)2 form has not been solved before. X-ray diffraction data for, the P2(1)2(1)2 form were collected at a wavelength of 1.54 A and data for, the I222 form were collected at three different wavelengths: 1.34, 1.07, and 0.98 A. The amount of anomalous signal from one Mn and eight S atoms, in these data sets varies from 1.24% to as low as 0.56%. The dual-space, direct-methods program SHELXD, run against the Bijvoet differences, gave a, clear solution of all anomalous scatterers for all data sets. The Mn, positions only were used for SAD phasing of all four data sets. ... [[http://ispc.weizmann.ac.il/pmbin/getpm?12777803 (full description)]]

==About this Structure==
1OAD is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Streptomyces_rubiginosus Streptomyces rubiginosus]] with MN, MG, MRD, MQD, TRS and MPD as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.5 5.3.1.5]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1OAD OCA]]. 

==Reference==
SAD manganese in two crystal forms of glucose isomerase., Ramagopal UA, Dauter M, Dauter Z, Acta Crystallogr D Biol Crystallogr. 2003 May;59(Pt 5):868-75. Epub 2003, Apr 25. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12777803 12777803]
[[Category: Single protein]]
[[Category: Streptomyces rubiginosus]]
[[Category: Dauter, M.]]
[[Category: Dauter, Z.]]
[[Category: Ramagopal, U.A.]]
[[Category: MG]]
[[Category: MN]]
[[Category: MPD]]
[[Category: MQD]]
[[Category: MRD]]
[[Category: TRS]]
[[Category: glucose isomerase]]
[[Category: isomerase]]
[[Category: xylose isomerase]]

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