View source for 1son

[[Image:1son.jpg|left|200px]]<br /><applet load="1son" size="450" color="white" frame="true" align="right" spinBox="true" 
caption="1son, resolution 2.55&Aring;" />
'''ADENYLOSUCCINATE SYNTHETASE IN COMPLEX WITH THE NATURAL FEEDBACK INHIBITOR AMP'''<br />

==Overview==
(+)-Hydantocidin, a recently discovered natural spironucleoside with, potent herbicidal activity, is shown to be a proherbicide that, after, phosphorylation at the 5' position, inhibits adenylosuccinate synthetase, an enzyme involved in de novo purine synthesis. The mode of binding of, hydantocidin 5'-monophosphate to the target enzyme was analyzed by, determining the crystal structure of the enzyme-inhibitor complex at 2.6-A, resolution. It was found that adenylosuccinate synthetase binds the, phosphorylated compound in the same fashion as it does adenosine, 5'-monophosphate, the natural feedback regulator of this enzyme. This work, provides the first crystal structure of a herbicide-target complex, reported to date.

==About this Structure==
1SON is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with AMP and BME as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Adenylosuccinate_synthase Adenylosuccinate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.4.4 6.3.4.4] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1SON OCA]. 

==Reference==
The mode of action and the structure of a herbicide in complex with its target: binding of activated hydantocidin to the feedback regulation site of adenylosuccinate synthetase., Fonne-Pfister R, Chemla P, Ward E, Girardet M, Kreuz KE, Honzatko RB, Fromm HJ, Schar HP, Grutter MG, Cowan-Jacob SW, Proc Natl Acad Sci U S A. 1996 Sep 3;93(18):9431-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8790347 8790347]
[[Category: Adenylosuccinate synthase]]
[[Category: Escherichia coli]]
[[Category: Single protein]]
[[Category: Cowan-Jacob, S.W.]]
[[Category: AMP]]
[[Category: BME]]
[[Category: gtp-hydrolyzing enzyme]]
[[Category: herbicide]]
[[Category: ligase]]
[[Category: purine nucleotide biosynthesis]]
[[Category: synthetase]]

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