View source for 2evj

[[Image:2evj.gif|left|200px]]<br /><applet load="2evj" size="450" color="white" frame="true" align="right" spinBox="true" 
caption="2evj, resolution 1.890&Aring;" />
'''Structure of an Ndt80-DNA complex (MSE mutant mA9C)'''<br />

==Overview==
The Saccharomyces cerevisiae transcription factor Ndt80 selectively binds, a DNA consensus sequence (the middle sporulation element [MSE]) to, activate gene expression after the successful completion of meiotic, recombination. Here we report the X-ray crystal structures of Ndt80 bound, to ten distinct MSE variants. Comparison of these structures with the, structure of Ndt80 bound to a consensus MSE reveals structural principles, that determine the DNA binding specificity of this transcription factor., The 5' GC-rich end of the MSE contains distinct 5'-YpG-3' steps that are, recognized by arginine side chains through a combination of hydrogen, bonding and cation-pi interactions. The 3' AT-rich region is recognized, via minor groove contacts that sterically exclude the N2 atom of GC base, pairs. The conformation of the AT-rich region is fixed by interactions, with the protein that favor recognition of poly(A)-poly(T) versus mixed AT, sequences through an avoidance of major groove steric clashes at 5'-ApT-3', steps.

==About this Structure==
2EVJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2EVJ OCA]. 

==Reference==
Principles of protein-DNA recognition revealed in the structural analysis of Ndt80-MSE DNA complexes., Lamoureux JS, Glover JN, Structure. 2006 Mar;14(3):555-65. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16531239 16531239]
[[Category: Saccharomyces cerevisiae]]
[[Category: Single protein]]
[[Category: Glover, J.N.]]
[[Category: Lamoureux, J.S.]]
[[Category: beta-barrel]]
[[Category: ig-fold transcription factor]]

''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 10:10:34 2007''