View source for 2tps

[[Image:2tps.gif|left|200px]]<br /><applet load="2tps" size="450" color="white" frame="true" align="right" spinBox="true" 
caption="2tps, resolution 1.25&Aring;" />
'''THIAMIN PHOSPHATE SYNTHASE'''<br />

==Overview==
The characterization of a three-gene operon (the thiC operon) at 331 min, which is involved in thiamine biosynthesis in Bacillus subtilis, is, described. The first gene in the operon is homologous to transcription, activators in the lysR family. The second and third genes (thiK and thiC), have been subcloned and overexpressed in Escherichia coli. ThiK (30 kDa), catalyzes the phosphorylation of 4-methyl-5-(beta-hydroxyethyl)thiazole., ThiC (27 kDa) catalyzes the substitution of the pyrophosphate of, 2-methyl-4-amino-5-hydroxymethylpyrimidine pyrophosphate by, 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate to yield thiamine, phosphate. Transcription of the thiC operon is not regulated by thiamine, or 2-methyl-4-amino-5-hydroxymethylpyrimidine and is only slightly, repressed by 4-methyl-5-(beta-hydroxyethyl)thiazole.

==About this Structure==
2TPS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis] with MG, TPS and POP as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Thiamine-phosphate_diphosphorylase Thiamine-phosphate diphosphorylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.3 2.5.1.3] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2TPS OCA]. 

==Reference==
Characterization of the Bacillus subtilis thiC operon involved in thiamine biosynthesis., Zhang Y, Taylor SV, Chiu HJ, Begley TP, J Bacteriol. 1997 May;179(9):3030-5. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9139923 9139923]
[[Category: Bacillus subtilis]]
[[Category: Single protein]]
[[Category: Thiamine-phosphate diphosphorylase]]
[[Category: Begley, T.P.]]
[[Category: Chiu, H.J.]]
[[Category: Ealick, S.E.]]
[[Category: Reddick, J.J.]]
[[Category: MG]]
[[Category: POP]]
[[Category: TPS]]
[[Category: thiamin biosynthesis]]
[[Category: tim barrel]]

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