View source for 3pcb

[[Image:3pcb.gif|left|200px]]<br />
<applet load="3pcb" size="450" color="white" frame="true" align="right" spinBox="true" 
caption="3pcb, resolution 2.19&Aring;" />
'''STRUCTURE OF PROTOCATECHUATE 3,4-DIOXYGENASE COMPLEXED WITH 3-HYDROXYBENZOATE'''<br />

==Overview==
Protocatechuate 3,4-dioxygenase (3,4-PCD) catalyzes the oxidative ring, cleavage of 3,4-dihydroxybenzoate to produce beta-carboxy-cis, cis-muconate. Crystal structures of Pseudomonas putida3,4-PCD [quaternary, structure of (alphabetaFe3+)12] complexed with seven competitive, inhibitors [3-hydroxyphenylacetate (MHP), 4-hydroxyphenylacetate (PHP), 3-hydroxybenzoate (MHB), 4-hydroxybenzoate (PHB), 3-fluoro-4-hydroxybenzoate (FHB), 3-chloro-4-hydroxybenzoate (CHB), and, 3-iodo-4-hydroxybenzoate (IHB)] are reported at 2.0-2.2 A resolution with, R-factors of 0. 0.159-0.179. The inhibitors bind in a narrow active site, crevasse lined with residues that provide a microenvironment that closely, matches the chemical characteristics of the inhibitors. This results in as, little as 20% ... [[http://ispc.weizmann.ac.il/pmbin/getpm?9254599 (full description)]]

==About this Structure==
3PCB is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]] with FE, BME and 3HB as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Oxidoreductase Oxidoreductase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.13.11.3 1.13.11.3]]. Structure known Active Sites: ACA, ACB, ACC, ACD, ACE, ACF, VEA, VEB, VEC, VED, VEE and VEF. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=3PCB OCA]]. 

==Reference==
Structures of competitive inhibitor complexes of protocatechuate 3,4-dioxygenase: multiple exogenous ligand binding orientations within the active site., Orville AM, Elango N, Lipscomb JD, Ohlendorf DH, Biochemistry. 1997 Aug 19;36(33):10039-51. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9254599 9254599]
[[Category: Protein complex]]
[[Category: Pseudomonas putida]]
[[Category: Elango, N.]]
[[Category: Lipscomb, J.D.]]
[[Category: Ohlendorf, D.H.]]
[[Category: Orville, A.M.]]
[[Category: 3HB]]
[[Category: BME]]
[[Category: FE]]
[[Category: dioxygenase]]
[[Category: iron]]
[[Category: metalloprotein]]
[[Category: nonheme]]
[[Category: oxidoreductase]]

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