View source for 10sm


==Importin-9 bound to ETS homologous factor (EHF)==
<StructureSection load='10sm' size='340' side='right'caption='[[10sm]], [[Resolution|resolution]] 3.50&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[10sm]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=10SM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=10SM FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.5&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=10sm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=10sm OCA], [https://pdbe.org/10sm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=10sm RCSB], [https://www.ebi.ac.uk/pdbsum/10sm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=10sm ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/EHF_HUMAN EHF_HUMAN] Transcriptional activator that may play a role in regulating epithelial cell differentiation and proliferation. May act as a repressor for a specific subset of ETS/AP-1-responsive genes and as a modulator of the nuclear response to mitogen-activated protein kinase signaling cascades. Binds to DNA sequences containing the consensus nucleotide core sequence GGAA. Involved in regulation of TNFRSF10B/DR5 expression through Ets-binding sequences on the TNFRSF10B/DR5 promoter. May contribute to development and carcinogenesis by acting as a tumor suppressor gene or anti-oncogene.<ref>PMID:10527851</ref> <ref>PMID:10644770</ref> <ref>PMID:11259407</ref> <ref>PMID:12444029</ref> <ref>PMID:17027647</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Protein trafficking between the cytoplasm and the nucleus is a fundamental process in eukaryotic cell biology. While linear nuclear localization signals (NLSs) are well characterized, many nuclear proteins lack a predictable NLS. Here, we identify the ETS domain, a DNA-binding winged-helix fold, from ETS family transcription factors as a structure-encoded NLS. We show that ETS domains mediate nuclear import through direct nanomolar affinity recognition by IPO9. Cryo-electron microscopy analysis of the EHF:IPO9 complex reveals that the IPO9 wraps around the ETS domain and engages structural features throughout the winged-helix fold. Biochemical studies demonstrate that the ETS domain DNA-binding helix is critical for importin recognition and for NLS activity in mammalian cells. Comparison of IPO9 bound to EHF and the histone H2A:H2B dimer reveals distinct interaction hotspots, illustrating how IPO9 employs unique combinatorial binding surfaces to accommodate structurally diverse cargos. These findings define a unique class of globular NLSs and highlight the adaptability of importins in recognizing distinct protein folds.

Importin-9 recognizes the winged-helix fold of ETS transcription factors to mediate nuclear import.,McConville M, Lankford K, Bernardes NE, Walterscheid A, Valadez C, Niesman A, Chook YM, Liszczak G Proc Natl Acad Sci U S A. 2026 May 5;123(18):e2536763123. doi: , 10.1073/pnas.2536763123. Epub 2026 May 1. PMID:42066049<ref>PMID:42066049</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 10sm" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Bernardes NE]]
[[Category: Chook YM]]
[[Category: Lankford K]]
[[Category: Liszczak G]]
[[Category: McConville M]]