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==Crystal structure of Mengla virus nucleoprotein bound by a cross-reactive anti-Marburg virus nanobody==
<StructureSection load='10zo' size='340' side='right'caption='[[10zo]], [[Resolution|resolution]] 1.65&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[10zo]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Dianlovirus_menglaense Dianlovirus menglaense] and [https://en.wikipedia.org/wiki/Lama_glama Lama glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=10ZO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=10ZO FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.65&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=10zo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=10zo OCA], [https://pdbe.org/10zo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=10zo RCSB], [https://www.ebi.ac.uk/pdbsum/10zo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=10zo ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A1Q1NMU1_9MONO A0A1Q1NMU1_9MONO] 
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== Publication Abstract from PubMed ==
Although Marburg and Mengla viruses occupy different geographies and genera of the family Filoviridae, genetic and host similarities suggest spillover potential for the latter. While Marburg virus causes transmissible and often fatal hemorrhagic fever in humans, the pathogenicity of Mengla virus is unknown. Understanding antibody cross-reactivity between the two viruses appears prudent in preparation for detecting the new virus and facilitating component-based studies of replication. Previously, while nanobodies to the monomeric nucleoprotein C-terminal domain (NPCTD) of Marburg virus recognized Mengla virus NPCTD, cross-reactivity was too weak to quantify monovalent equilibrium concentrations. Here, using oligomeric NP in a nanobody-driven sandwich assay, the cross-reactivity deficit was essentially negated, suggesting we would be able to detect both viruses equally. Curious as to why monovalent reactivity was so disparate, we crystallized the Mengla virus NPCTD-nanobody complex for X-ray crystal structure determination. Comparative analysis of the antibody-antigen interfaces revealed bonded and nonbonded opportunities at one location in the Marburg complex that were absent in the Mengla complex. Mutagenesis of the NPCTDs, to make Marburg more Mengla-like (H690N) and Mengla more Marburg-like (N692H), resulted in dramatic ablation and restoration of nanobody binding, respectively, via changes in off-rates. Similar trends were observed for the more recently discovered Dehong virus, and dimeric enzymatic and fluorescent reporter fusions improved NP recognition potency within blotting and cell probing assays. Understanding the structural basis for cross-reactivity helps predict the likelihood of detecting viral variants based upon genomic sequence information and can inform the design of antibodies with broader recognition potential.

Crystal Structure of Mengla Virus Nucleoprotein Bound by a Poorly Cross-Reactive Anti-Marburg Virus Nanobody Highlights a Single Amino Acid Affinity Switch, a Feature Also Evident in Dehong Virus.,Sherwood LJ, Taylor AB, Olsen SK, Hayhurst A ACS Infect Dis. 2026 Mar 13;12(3):1104-1121. doi: 10.1021/acsinfecdis.5c00920. , Epub 2026 Feb 27. PMID:41760080<ref>PMID:41760080</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 10zo" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Dianlovirus menglaense]]
[[Category: Lama glama]]
[[Category: Large Structures]]
[[Category: Olsen SK]]
[[Category: Taylor AB]]