View source for 12qa


==Crystal structure of CM10 Fab in complex with an orthomarburgvirus GP2 peptide==
<StructureSection load='12qa' size='340' side='right'caption='[[12qa]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[12qa]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Macaca_mulatta Macaca mulatta] and [https://en.wikipedia.org/wiki/Orthomarburgvirus_marburgense Orthomarburgvirus marburgense]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=12QA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=12QA FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=12qa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=12qa OCA], [https://pdbe.org/12qa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=12qa RCSB], [https://www.ebi.ac.uk/pdbsum/12qa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=12qa ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/VGP_MABVR VGP_MABVR] GP1 is responsible for binding to the receptor(s) on target cells. Interacts with CD209/DC-SIGN and CLEC4M/DC-SIGNR which act as cofactors for virus entry into the host cell. Binding to CD209 and CLEC4M, which are respectively found on dendritic cells (DCs), and on endothelial cells of liver sinusoids and lymph node sinuses, facilitate infection of macrophages and endothelial cells. These interactions not only facilitate virus cell entry, but also allow capture of viral particles by DCs and subsequent transmission to susceptible cells without DCs infection (trans infection) (By similarity).  GP2 acts as a class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in GP2, releasing the fusion hydrophobic peptide (By similarity).
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Macaca mulatta]]
[[Category: Orthomarburgvirus marburgense]]
[[Category: Janus BM]]
[[Category: Metcalf M]]
[[Category: Niyongabo A]]
[[Category: Ofek G]]