View source for 13jq


==Structure of Thermus thermophilus Mfd==
<StructureSection load='13jq' size='340' side='right'caption='[[13jq]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[13jq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=13JQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=13JQ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=13jq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=13jq OCA], [https://pdbe.org/13jq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=13jq RCSB], [https://www.ebi.ac.uk/pdbsum/13jq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=13jq ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q5SJW3_THET8 Q5SJW3_THET8] Couples transcription and DNA repair by recognizing RNA polymerase (RNAP) stalled at DNA lesions. Mediates ATP-dependent release of RNAP and its truncated transcript from the DNA, and recruitment of nucleotide excision repair machinery to the damaged site.[HAMAP-Rule:MF_00969]
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== Publication Abstract from PubMed ==
Mfd, the canonical bacterial transcription-repair coupling factor, is a highly conserved ATP-dependent DNA translocase with a complex architecture undergoing major rearrangements during its functional cycle. These changes regulate its ATPase and motor activities and are tuned by Mfd interactions with DNA, RNA polymerase and the UvrA subunit of the nucleotide excision repair excinuclease, Uvr(A)BC. Due to its role in accelerating molecular evolution and the development of antibiotic resistance, Mfd is also rapidly emerging as a prime target for the development of anti-evolution drugs to be administered in combination with narrow-spectrum antibiotics to prevent the rise of resistance and combat infection over a wider time window. Here we present the crystal structure of Thermus thermophilus Mfd in its nucleotide-free state. We note the pronounced disorder of the N-terminal UvrB homology module, which was previously seen in Escherichia coli to be engaged in a "clamp" interaction with the C-terminal domain, resulting in autoinhibition of its ATP-dependent functions. Thus, we conclude that the autoinhibitory interdomain interactions, such as the clamp, are not a universal feature of transcription-repair coupling factors. Consistent with this, Thermus thermophilus Mfd, unlike Escherichia coli Mfd, translocates robustly on DNA even in the absence of RNA polymerase and displays DNA binding that is largely nucleotide independent. Our work brings mechanistic insight into the species-specific differences in Mfd structure and function and provides a structural framework for the design of anti-evolution drugs to combat antimicrobial resistance.

Autoinhibition is not a universal feature of transcription-repair coupling factors.,Brugger C, Suhanovsky MM, Son J, Deaconescu AM J Biol Chem. 2026 Aug;302(8):113280. doi: 10.1016/j.jbc.2026.113280. Epub 2026 , Aug 1. PMID:42331104<ref>PMID:42331104</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 13jq" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Large Structures]]
[[Category: Thermus thermophilus HB8]]
[[Category: Brugger C]]
[[Category: Deaconescu AM]]
[[Category: Suhanovsky MM]]