View source for 1bbx


==NON-SPECIFIC PROTEIN-DNA INTERACTIONS IN THE SSO7D-DNA COMPLEX, NMR, 1 STRUCTURE==
<StructureSection load='1bbx' size='340' side='right'caption='[[1bbx]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1bbx]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharolobus_solfataricus Saccharolobus solfataricus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BBX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BBX FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1bbx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bbx OCA], [https://pdbe.org/1bbx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1bbx RCSB], [https://www.ebi.ac.uk/pdbsum/1bbx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1bbx ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/DN7D_SACS2 DN7D_SACS2] Can constrain negative DNA supercoils. May be involved in maintaining the integrity of the genome at high temperature (By similarity). Stimulates the Holliday junction cleavage activity of Hjc (PubMed:11709558).[UniProtKB:P61990]<ref>PMID:11709558</ref> 
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== Publication Abstract from PubMed ==
Many biochemical processes, including DNA packing, maintenance and control, rely on non-sequence specific protein-DNA interactions. Nonspecific DNA-binding proteins have evolved to tolerate a wide range of DNA sequences, yet bind with a respectable affinity. The nonspecific binding requirement is in contrast to that imposed on, for example, transcription factors and implies a different structural basis for the biomolecular recognition process. To address this issue, and the mechanism for archaeal DNA packing, we determined the structure of the Sso7d protein from Sulfolobus solfataricus in complex with DNA. Sso7d binds DNA by placing a triple-stranded beta-sheet across the DNA minor groove. The protein is anchored in this position by the insertion of hydrogen bond-donating side chains into the groove and additionally stabilized by electrostatic and non-polar interactions with the DNA backbone. This structure explains how strong binding can be achieved independent of DNA sequence. Sso7d binding also distorts the DNA conformation and introduces significant unwinding of the helix. This effect suggests a mechanism for DNA packing in Sulfolobus based on negative DNA supercoiling.

Architecture of nonspecific protein-DNA interactions in the Sso7d-DNA complex.,Agback P, Baumann H, Knapp S, Ladenstein R, Hard T Nat Struct Biol. 1998 Jul;5(7):579-84. PMID:9665172<ref>PMID:9665172</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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== References ==
<references/>
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</StructureSection>
[[Category: Large Structures]]
[[Category: Saccharolobus solfataricus]]
[[Category: Agback P]]
[[Category: Baumann H]]
[[Category: Hard T]]
[[Category: Knapp S]]
[[Category: Ladenstein R]]