View source for 1byp


==E43K,D44K DOUBLE MUTANT PLASTOCYANIN FROM SILENE==
<StructureSection load='1byp' size='340' side='right'caption='[[1byp]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1byp]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Silene_latifolia_subsp._alba Silene latifolia subsp. alba]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BYP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BYP FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.75&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1byp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1byp OCA], [https://pdbe.org/1byp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1byp RCSB], [https://www.ebi.ac.uk/pdbsum/1byp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1byp ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/PLAS_SILLB PLAS_SILLB] Participates in electron transfer between P700 and the cytochrome b6-f complex in photosystem I.<ref>PMID:10220581</ref> 
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/by/1byp_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1byp ConSurf].
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== Publication Abstract from PubMed ==
Plastocyanin functions as an electron carrier between the cytochrome b6f complex and photosystem I. The crystal structures of the wild-type and E43K/D44K double mutant from the higher plant, Silene, have been determined at 2.0 and 1.75 A resolution, respectively. The wild-type plastocyanin comprises two monomers per asymmetric unit, one of which shows the unusually great distance between the copper ion and the Ndelta1 atom of H87 because of the hydrogen bond network formation between H87 and symmetry-related G10. The root mean square deviation for Ca atoms between the wild-type and mutant plastocyanins is 0.44 A, however, the electrostatic potential maps of their molecular surfaces are remarkably different. The low electron-transfer rate in the E43K/D44K mutant results from the hindrance of electrostatic interactions, not from the structural change due to the mutation.

Crystal structures of wild-type and mutant plastocyanins from a higher plant, Silene.,Sugawara H, Inoue T, Li C, Gotowda M, Hibino T, Takabe T, Kai Y J Biochem. 1999 May;125(5):899-903. PMID:10220581<ref>PMID:10220581</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 1byp" style="background-color:#fffaf0;"></div>

==See Also==
*[[Plastocyanin 3D structures|Plastocyanin 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Silene latifolia subsp. alba]]
[[Category: Gotowda M]]
[[Category: Hibino T]]
[[Category: Inoue T]]
[[Category: Kai Y]]
[[Category: Li C]]
[[Category: Sugawara H]]
[[Category: Takabe T]]