View source for 1dbd


==E2 DNA-BINDING DOMAIN FROM PAPILLOMAVIRUS BPV-1==
<StructureSection load='1dbd' size='340' side='right'caption='[[1dbd]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1dbd]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Deltapapillomavirus_4 Deltapapillomavirus 4]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DBD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1DBD FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1dbd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dbd OCA], [https://pdbe.org/1dbd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1dbd RCSB], [https://www.ebi.ac.uk/pdbsum/1dbd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1dbd ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/VE2_BPV1 VE2_BPV1] E2 regulates viral transcription and DNA replication. Binds to the E2RE response element (5'-ACCNNNNNNGGT-3') present in multiple copies in the regulatory region. Can either activate or repress transcription depending on E2RE's position with regards to proximal promoter elements. Repression occurs by sterically hindering the assembly of the transcription initiation complex. The E1-E2 complex binds to the origin of DNA replication.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/db/1dbd_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1dbd ConSurf].
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== Publication Abstract from PubMed ==
Papillomaviral E2 proteins participate in viral DNA replication and transcriptional regulation. We have solved the solution structure of the DNA-binding domain of the E2 protein from bovine papillomavirus (BPV-1). The structure calculation used 2222 distance and 158 dihedral angle restraints for the homodimer (202 residues in total), which were derived from homonuclear and heteronuclear multidimensional nuclear magnetic resonance (NMR) spectroscopic data. The root-mean-square deviation for structured regions of the monomer when superimposed to the average is 0.73 +/- 0.10 A for backbone atoms and 1.42 +/- 0.16 A for heavy atoms. The 101 residue construct used in this study (residues 310-410) is about 4.5 kcal/mol more stable than a minimal domain comprising the C-terminal 85 amino acid residues (residues 326-410). The structure of the core domain contained within BPV-1 E2 is similar to the corresponding regions of other papilloma viral E2 proteins. Here, however, the extra N-terminal 16 residues form a flap that covers a cavity at the dimer interface and play a role in DNA binding. Interactions between residues in the N-terminal extension and the core domain correlate with the greater stability of the longer form of the protein relative to the minimal domain.

Structural correlates for enhanced stability in the E2 DNA-binding domain from bovine papillomavirus.,Veeraraghavan S, Mello CC, Androphy EJ, Baleja JD Biochemistry. 1999 Dec 7;38(49):16115-24. PMID:10587434<ref>PMID:10587434</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 1dbd" style="background-color:#fffaf0;"></div>

==See Also==
*[[Regulatory protein E2|Regulatory protein E2]]
== References ==
<references/>
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</StructureSection>
[[Category: Deltapapillomavirus 4]]
[[Category: Large Structures]]
[[Category: Androphy EJ]]
[[Category: Baleja JD]]
[[Category: Mello CC]]
[[Category: Veeraraghavan S]]