View source for 1dx8


==Rubredoxin from Guillardia theta==
<StructureSection load='1dx8' size='340' side='right'caption='[[1dx8]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1dx8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Guillardia_theta Guillardia theta]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DX8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1DX8 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1dx8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dx8 OCA], [https://pdbe.org/1dx8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1dx8 RCSB], [https://www.ebi.ac.uk/pdbsum/1dx8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1dx8 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q9XG40_GUITH Q9XG40_GUITH] 
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/dx/1dx8_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1dx8 ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The rubredoxin from the cryptomonad Guillardia theta is one of the first examples of a rubredoxin encoded in a eukaryotic organism. The structure of a soluble zinc-substituted 70-residue G. theta rubredoxin lacking the membrane anchor and the thylakoid targeting sequence was determined by multidimensional heteronuclear NMR, representing the first three-dimensional (3D) structure of a eukaryotic rubredoxin. For the structure calculation a strategy was applied in which information about hydrogen bonds was directly inferred from a long-range HNCO experiment, and the dynamics of the protein was deduced from heteronuclear nuclear Overhauser effect data and exchange rates of the amide protons. The structure is well defined, exhibiting average root-mean-square deviations of 0.21 A for the backbone heavy atoms and 0.67 A for all heavy atoms of residues 7-56, and an increased flexibility toward the termini. The structure of this core fold is almost identical to that of prokaryotic rubredoxins. There are, however, significant differences with respect to the charge distribution at the protein surface, suggesting that G. theta rubredoxin exerts a different physiological function compared to the structurally characterized prokaryotic rubredoxins. The amino-terminal residues containing the putative signal peptidase recognition/cleavage site show an increased flexibility compared to the core fold, but still adopt a defined 3D orientation, which is mainly stabilized by nonlocal interactions to residues of the carboxy-terminal region. This orientation might reflect the structural elements and charge pattern necessary for correct signal peptidase recognition of the G. theta rubredoxin precursor.

Solution structure of a zinc substituted eukaryotic rubredoxin from the cryptomonad alga Guillardia theta.,Schweimer K, Hoffmann S, Wastl J, Maier UG, Rosch P, Sticht H Protein Sci. 2000 Aug;9(8):1474-86. PMID:10975569<ref>PMID:10975569</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 1dx8" style="background-color:#fffaf0;"></div>

==See Also==
*[[Rubredoxin 3D structures|Rubredoxin 3D structures]]
*[[Rubredoxin PDB structures|Rubredoxin PDB structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Guillardia theta]]
[[Category: Large Structures]]
[[Category: Hoffmann S]]
[[Category: Maier UG]]
[[Category: Roesch P]]
[[Category: Schweimer K]]
[[Category: Sticht H]]
[[Category: Wastl J]]