View source for 1eht


==THEOPHYLLINE-BINDING RNA IN COMPLEX WITH THEOPHYLLINE, NMR, 10 STRUCTURES==
<StructureSection load='1eht' size='340' side='right'caption='[[1eht]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1eht]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EHT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EHT FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=TEP:THEOPHYLLINE'>TEP</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1eht FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eht OCA], [https://pdbe.org/1eht PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1eht RCSB], [https://www.ebi.ac.uk/pdbsum/1eht PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1eht ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
To visualize the interplay of RNA structural interactions in a ligand binding site, we have determined the solution structure of a high affinity RNA-theophylline complex using NMR spectroscopy. The structure provides insight into the ability of this in vitro selected RNA to discriminate theophylline from the structurally similar molecule caffeine. Numerous RNA structural motifs combine to form a well-ordered binding pocket where an intricate network of hydrogen bonds and stacking interactions lock the theophylline into the complex. Two internal loops interact to form the binding site which consists of a sandwich of three base triples. The complex also contains novel base-zipper and 1-3-2 stacking motifs, in addition to an adenosine platform and a reversed sugar. An important feature of the RNA is that many of the conserved core residues participate in multiple overlapping tertiary interactions. This complex illustrates how interlocking structural motifs can be assembled into a highly specific ligand-binding site that possesses high levels of affinity and molecular discrimination.

Interlocking structural motifs mediate molecular discrimination by a theophylline-binding RNA.,Zimmermann GR, Jenison RD, Wick CL, Simorre JP, Pardi A Nat Struct Biol. 1997 Aug;4(8):644-9. PMID:9253414<ref>PMID:9253414</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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== References ==
<references/>
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</StructureSection>
[[Category: Large Structures]]
[[Category: Pardi A]]
[[Category: Zimmermann GR]]