View source for 1ie2


==Solution Structure of an In Vitro Selected RNA which is Sequence Specifically Recognized by RBD12 of Hamster Nucleolin.sNRE (anti)==
<StructureSection load='1ie2' size='340' side='right'caption='[[1ie2]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1ie2]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IE2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1IE2 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ie2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ie2 OCA], [https://pdbe.org/1ie2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ie2 RCSB], [https://www.ebi.ac.uk/pdbsum/1ie2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ie2 ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
Nucleolin is an abundant nucleolar protein which is essential for ribosome biogenesis. The first two of its four tandem RNA-binding domains (RBD12) specifically recognize a stem-loop structure containing a conserved UCCCGA sequence in the loop called the nucleolin-recognition element (NRE). We have determined the structure of the consensus SELEX NRE (sNRE) by NMR spectroscopy. In both the free and bound RNA the top part of the stem forms a loop E (or S-turn) motif. In the absence of protein, the structure of the hairpin loop is not well defined due to conformational heterogeneity, and appears to be in equilibrium between two families of conformations. Titrations of RBD1, RBD2, and RBD12 with the sNRE show that specific binding requires RBD12. In complex with RBD12, the hairpin loop interacts specifically with the protein and adopts a well-defined structure which shares some of the features of the free form. The loop E motif also has specific interactions with the protein. Implications of these findings for the mechanism of recognition of RNA structures by modular proteins are discussed.

Recognition of pre-formed and flexible elements of an RNA stem-loop by nucleolin.,Bouvet P, Allain FH, Finger LD, Dieckmann T, Feigon J J Mol Biol. 2001 Jun 8;309(3):763-75. PMID:11397095<ref>PMID:11397095</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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== References ==
<references/>
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</StructureSection>
[[Category: Large Structures]]
[[Category: Allain FH-T]]
[[Category: Bouvet P]]
[[Category: Dieckmann T]]
[[Category: Feigon J]]
[[Category: Finger LD]]