View source for 1ixd


==Solution structure of the CAP-GLY domain from human cylindromatosis tomour-suppressor CYLD==
<StructureSection load='1ixd' size='340' side='right'caption='[[1ixd]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1ixd]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IXD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1IXD FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ixd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ixd OCA], [https://pdbe.org/1ixd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ixd RCSB], [https://www.ebi.ac.uk/pdbsum/1ixd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ixd ProSAT], [https://www.topsan.org/Proteins/RSGI/1ixd TOPSAN]</span></td></tr>
</table>
== Disease ==
[https://www.uniprot.org/uniprot/CYLD_HUMAN CYLD_HUMAN] Defects in CYLD are the cause of familial cylindromatosis (FCYL) [MIM:[https://omim.org/entry/132700 132700]; also known as Ancell-Spiegler cylindromas or turban tumor syndrome or dermal eccrine cylindromatosis. CYLD is an autosomal dominant and highly tumor type-specific disorder. The tumors (known as cylindromas because of their characteristic microscopic architecture) are believed to arise from or recapitulate the appearance of the eccrine or apocrine cells of the skin that secrete sweat and scent respectively. Cylindromas arise predominantly in hairy parts of the body with approximately 90% on the head and neck. The development of a confluent mass which may ulcerate or become infected has led to the designation 'turban tumor syndrome'. The skin tumors show differentiation in the direction of hair structures, hence the synonym trichoepithelioma.<ref>PMID:12190880</ref> <ref>PMID:16922728</ref>   Defects in CYLD are the cause of multiple familial trichoepithelioma type 1 (MFT1) [MIM:[https://omim.org/entry/601606 601606]; also known as epithelioma adenoides cysticum of Brooke (EAC) or hereditary multiple benign cystic epithelioma or Brooke-Fordyce trichoepitheliomas. MFT1 is an autosomal dominant dermatosis characterized by the presence of many skin tumors predominantly on the face. Since histologic examination shows dermal aggregates of basaloid cells with connection to or differentiation toward hair follicles, this disorder has been thought to represent a benign hamartoma of the pilosebaceous apparatus. Trichoepitheliomas can degenerate into basal cell carcinoma.<ref>PMID:16922728</ref> <ref>PMID:14632188</ref> <ref>PMID:16307661</ref>   Defects in CYLD are the cause of Brooke-Spiegler syndrome (BRSS) [MIM:[https://omim.org/entry/605041 605041]. BRSS is an autosomal dominant disorder characterized by the appearance of multiple skin appendage tumors such as cylindroma, trichoepithelioma, and spiradenoma. These tumors are typically located in the head and neck region, appear in early adulthood, and gradually increase in size and number throughout life.<ref>PMID:12190880</ref> <ref>PMID:14632188</ref> <ref>PMID:12950348</ref> <ref>PMID:15854031</ref> 
== Function ==
[https://www.uniprot.org/uniprot/CYLD_HUMAN CYLD_HUMAN] Protease that specifically cleaves 'Lys-63'-linked polyubiquitin chains. Has endodeubiquitinase activity. Plays an important role in the regulation of pathways leading to NF-kappa-B activation. Contributes to the regulation of cell survival, proliferation and differentiation via its effects on NF-kappa-B activation. Negative regulator of Wnt signaling. Inhibits HDAC6 and thereby promotes acetylation of alpha-tubulin and stabilization of microtubules. Plays a role in the regulation of microtubule dynamics, and thereby contributes to the regulation of cell proliferation, cell polarization, cell migration, and angiogenesis. Required for normal cell cycle progress and normal cytokinesis. Inhibits nuclear translocation of NF-kappa-B. Plays a role in the regulation of inflammation and the innate immune response, via its effects on NF-kappa-B activation. Dispensable for the maturation of intrathymic natural killer cells, but required for the continued survival of immature natural killer cells. Negatively regulates TNFRSF11A signaling and osteoclastogenesis (By similarity).<ref>PMID:12917689</ref> <ref>PMID:12917690</ref> <ref>PMID:12917691</ref> <ref>PMID:14676304</ref> <ref>PMID:15870263</ref> <ref>PMID:17495026</ref> <ref>PMID:18636086</ref> <ref>PMID:18222923</ref> <ref>PMID:20194890</ref> <ref>PMID:19893491</ref> <ref>PMID:20227366</ref> <ref>PMID:18313383</ref> 
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ix/1ixd_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ixd ConSurf].
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== Publication Abstract from PubMed ==
CYLD was originally identified as the human familial cylindromatosis tumor suppressor. Recently, it was reported that CYLD directly interacts with NEMO/IKKgamma and TRAF2 in the NF-kappaB signaling pathway. The two proteins bind to a region of CYLD that contains a Cys-box motif and the third cytoskeleton-associated protein-glycine conserved (CAP-Gly) domain. Here we report that the third CAP-Gly domain of CYLD specifically interacts with one of the two proline-rich sequences of NEMO/IKKgamma. The tertiary structure of the CAP-Gly domain shares the five-stranded beta sheet topology with the SH3 domain, which is well known as a proline-rich sequence-recognition domain. However, chemical shift mapping revealed that the peptide binding site of the CAP-Gly domain is formed without the long peptide binding loop characteristic of the SH3 domain. Therefore, CAP-Gly is likely to be a novel proline-rich sequence binding domain with a mechanism different from that of the SH3 domain.

The CAP-Gly domain of CYLD associates with the proline-rich sequence in NEMO/IKKgamma.,Saito K, Kigawa T, Koshiba S, Sato K, Matsuo Y, Sakamoto A, Takagi T, Shirouzu M, Yabuki T, Nunokawa E, Seki E, Matsuda T, Aoki M, Miyata Y, Hirakawa N, Inoue M, Terada T, Nagase T, Kikuno R, Nakayama M, Ohara O, Tanaka A, Yokoyama S Structure. 2004 Sep;12(9):1719-28. PMID:15341735<ref>PMID:15341735</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Kigawa T]]
[[Category: Koshiba S]]
[[Category: Saito K]]
[[Category: Yokoyama S]]