View source for 1ksx


==Crystal Structures of Two Intermediates in the Assembly of the Papillomavirus Replication Initiation Complex==
<StructureSection load='1ksx' size='340' side='right'caption='[[1ksx]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1ksx]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Bovine_papillomavirus Bovine papillomavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KSX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KSX FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.2&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ksx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ksx OCA], [https://pdbe.org/1ksx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ksx RCSB], [https://www.ebi.ac.uk/pdbsum/1ksx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ksx ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/VE1_BPV1 VE1_BPV1] ATP-dependent DNA helicase required for initiation of viral DNA replication. It forms a complex with the viral E2 protein. The E1-E2 complex binds to the replication origin which contains binding sites for both proteins.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ks/1ksx_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ksx ConSurf].
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== Publication Abstract from PubMed ==
Initiation of DNA replication of the papillomavirus genome is a multi-step process involving the sequential loading of viral E1 protein subunits onto the origin of replication. Here we have captured structural snapshots of two sequential steps in the assembly process. Initially, an E1 dimer binds to adjacent major grooves on one face of the double helix; a second dimer then binds to another face of the helix. Each E1 monomer has two DNA-binding modules: a DNA-binding loop, which binds to one DNA strand and a DNA-binding helix, which binds to the opposite strand. The nature of DNA binding suggests a mechanism for the transition between double- and single-stranded DNA binding that is implicit in the progression to a functional helicase.

Crystal structures of two intermediates in the assembly of the papillomavirus replication initiation complex.,Enemark EJ, Stenlund A, Joshua-Tor L EMBO J. 2002 Mar 15;21(6):1487-96. PMID:11889054<ref>PMID:11889054</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 1ksx" style="background-color:#fffaf0;"></div>

==See Also==
*[[Replication protein E1|Replication protein E1]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Bovine papillomavirus]]
[[Category: Large Structures]]
[[Category: Enemark EJ]]
[[Category: Joshua-Tor L]]
[[Category: Stenlund A]]