View source for 1luz


==Crystal Structure of the K3L Protein From Vaccinia Virus (Wisconsin Strain)==
<StructureSection load='1luz' size='340' side='right'caption='[[1luz]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1luz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Vaccinia_virus_WR Vaccinia virus WR]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LUZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LUZ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1luz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1luz OCA], [https://pdbe.org/1luz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1luz RCSB], [https://www.ebi.ac.uk/pdbsum/1luz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1luz ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/PG041_VACCW PG041_VACCW] Viral mimic of EIF2S1/eIF-2alpha that acts as a pseudosubstrate for EIF2AK2/PKR kinase (PubMed:8099586, PubMed:9199350). Inhibits therefore EIF2S1/eIF-2alpha phosphorylation by host EIF2AK2/PKR kinase and prevents protein synthesis shutoff (PubMed:8099586, PubMed:9199350). Determinant of host species specificity (PubMed:32056708).<ref>PMID:32056708</ref> <ref>PMID:8099586</ref> <ref>PMID:9199350</ref> 
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/lu/1luz_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1luz ConSurf].
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== Publication Abstract from PubMed ==
The vaccinia virus protein K3L subverts the mammalian antiviral defense mechanism by inhibiting the RNA-dependent protein kinase PKR. K3L is a structural mimic of PKR's natural substrate, the translation initiation factor eIF2alpha. To further our understanding of K3L inhibitory function and PKR substrate recognition, we have solved the 1.8 A X-ray crystal structure of K3L. The structure consists of a five-strand beta barrel with an intervening helix insert region similar in topology to the functionally divergent S1 domain. Mutational analysis identifies two proximal regions of the K3L structure as possessing specialized PKR binding and inhibitory function. Further analysis reveals that PKR dimerization composes a key switch that regulates both its catalytic activation and its molecular recognition of K3L and eIF2alpha.

X-ray crystal structure and functional analysis of vaccinia virus K3L reveals molecular determinants for PKR subversion and substrate recognition.,Dar AC, Sicheri F Mol Cell. 2002 Aug;10(2):295-305. PMID:12191475<ref>PMID:12191475</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 1luz" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Vaccinia virus WR]]
[[Category: Dar AC]]
[[Category: Sicheri F]]