View source for 1mis


==STRUCTURE OF RNA (5'-R(GP*CP*GP*GP*AP*CP*GP*C)-3') ANTI-PARALLEL RNA DUPLEX WITH TANDEM G:A MISMATCHES, NMR, MINIMIZED AVERAGE STRUCTURE==
<StructureSection load='1mis' size='340' side='right'caption='[[1mis]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1mis]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MIS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MIS FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mis FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mis OCA], [https://pdbe.org/1mis PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mis RCSB], [https://www.ebi.ac.uk/pdbsum/1mis PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mis ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
The three-dimensional solution structure of the RNA self-complementary duplex [sequence: see text] was derived from two-dimensional NMR and the iterative relaxation matrix approach. Each GA mismatch forms two hydrogen bonds: A-NH6 to G-O6 and A-N1 to G-NH1 (imino). This is the first three-dimensional RNA structure with imino hydrogen-bonded tandem GA mismatches. This GA structure is totally different from the sheared tandem GA structure in [sequence: see text] which also has two hydrogen bonds: A-N7 to G-NH2 and A-NH6 to G-N3 [SantaLucia, J., Jr., &amp; Turner, D. H. (1993) Biochemistry 32, 12612-12623]. In particular, the sheared and imino GA mismatches produce a narrowing and widening of the backbone, respectively. The results show that substitutions of Watson-Crick base pairs can have dramatic effects on the three-dimensional structures of adjacent non-Watson-Crick paired regions; i.e., the structure depends on sequence context. Thus compensating substitutions in site-directed mutagenesis experiments may not always restore biological activities.

Solution structure of (rGCGGACGC)2 by two-dimensional NMR and the iterative relaxation matrix approach.,Wu M, Turner DH Biochemistry. 1996 Jul 30;35(30):9677-89. PMID:8703939<ref>PMID:8703939</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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== References ==
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[[Category: Large Structures]]
[[Category: Turner DH]]
[[Category: Wu M]]