View source for 1qes


==TANDEM GU MISMATCHES IN RNA, NMR, 30 STRUCTURES==
<StructureSection load='1qes' size='340' side='right'caption='[[1qes]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1qes]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QES OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QES FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1qes FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qes OCA], [https://pdbe.org/1qes PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1qes RCSB], [https://www.ebi.ac.uk/pdbsum/1qes PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1qes ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
The symmetric, tandem GU mismatch motifs, and , which only differ in the mismatch order, have an average difference in thermodynamic stability of 2 kcal/mol at 37 degrees C. Thermodynamic studies of duplexes containing these motifs indicate the effect is largely localized to the mismatches and adjacent base pairs. The three-dimensional structures of two representative duplexes, (rGGAGUUCC)2 and (rGGAUGUCC)2, were determined by two-dimensional NMR and a simulated annealing protocol. Local deviations are similar to other intrahelical GU mismatches with little effect on backbone torsion angles and a slight overtwisting between the base pair 5' of the G of the mismatch and the mismatch itself. Comparisons of the resulting stacking patterns along with electrostatic potential maps suggest that interactions between highly negative electrostatic regions between base pairs may play a role in the observed thermodynamic differences.

Investigation of the structural basis for thermodynamic stabilities of tandem GU wobble pairs: NMR structures of (rGGAGUUCC)2 and (rGGAUGUCC)2.,McDowell JA, He L, Chen X, Turner DH Biochemistry. 1997 Jul 1;36(26):8030-8. PMID:9201950<ref>PMID:9201950</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 1qes" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Chen X]]
[[Category: He L]]
[[Category: Mcdowell JA]]
[[Category: Turner DH]]