View source for 1scl


==THE SARCIN-RICIN LOOP, A MODULAR RNA==
<StructureSection load='1scl' size='340' side='right'caption='[[1scl]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1scl]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SCL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SCL FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1scl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1scl OCA], [https://pdbe.org/1scl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1scl RCSB], [https://www.ebi.ac.uk/pdbsum/1scl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1scl ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
The sarcin/ricin loop is a highly conserved sequence found in the RNA of all large ribosomal subunits. The cytotoxins alpha-sarcin and ricin both inactivate ribosomes by cleaving a single bond in that loop. Once it has been attacked, ribosomes no longer interact with elongation factors properly, and translation stops. We have determined the conformation of the sarcin/ricin loop by multinuclear NMR spectroscopy using E73, a 29-nucleotide RNA that has the sarcin/ricin loop sequence and that is sensitive to both toxins in vitro. The sarcin/ricin loop has a compact structure that contains several purine.purine base pairs, a GAGA tetraloop, and a bulged guanosine adjacent to a reverse Hoogsteen A.U pair. It is stabilized by an unusual set of cross-strand base-stacking interactions and imino proton to phosphate oxygen hydrogen bonds. In addition to having interesting structural features, this model explains many of the biochemical observations made about the loop's structure and its reactivity with cytotoxins, and it sheds light on the loop's interactions with elongation factors.

The conformation of the sarcin/ricin loop from 28S ribosomal RNA.,Szewczak AA, Moore PB, Chang YL, Wool IG Proc Natl Acad Sci U S A. 1993 Oct 15;90(20):9581-5. PMID:8415744<ref>PMID:8415744</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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== References ==
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</StructureSection>
[[Category: Large Structures]]
[[Category: Rattus norvegicus]]
[[Category: Moore PB]]
[[Category: Szewczak AA]]