View source for 1wlp


==Solution Structure Of The P22Phox-P47Phox Complex==
<StructureSection load='1wlp' size='340' side='right'caption='[[1wlp]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1wlp]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WLP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1WLP FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1wlp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wlp OCA], [https://pdbe.org/1wlp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1wlp RCSB], [https://www.ebi.ac.uk/pdbsum/1wlp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1wlp ProSAT]</span></td></tr>
</table>
== Disease ==
[https://www.uniprot.org/uniprot/CY24A_HUMAN CY24A_HUMAN] Defects in CYBA are a cause of chronic granulomatous disease autosomal recessive cytochrome-b-negative (ARCGD) [MIM:[https://omim.org/entry/233690 233690]. Chronic granulomatous disease is a genetically heterogeneous disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life-threatening bacterial/fungal infections.<ref>PMID:2243141</ref> <ref>PMID:1415254</ref> <ref>PMID:1763037</ref> <ref>PMID:8168815</ref> <ref>PMID:7964505</ref> <ref>PMID:10910929</ref> <ref>PMID:10759707</ref> <ref>PMID:10914676</ref> <ref>PMID:18422995</ref> 
== Function ==
[https://www.uniprot.org/uniprot/CY24A_HUMAN CY24A_HUMAN] Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. Associates with NOX3 to form a functional NADPH oxidase constitutively generating superoxide.<ref>PMID:15824103</ref> 
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wl/1wlp_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1wlp ConSurf].
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== Publication Abstract from PubMed ==
The phagocyte NADPH oxidase plays a crucial role in host defense against microbial infections by generating reactive oxygen species. It is a multisubunit enzyme composed of membrane-bound flavocytochrome b558 as well as cytosolic components, including p47phox, which is essential for assembly of the complex. When phagocytes are activated, the cytosolic components of the NADPH oxidase translocate to flavocytochrome b558 due to binding of the tandem Src homology 3 (SH3) domains of p47phox to a proline-rich region in p22phox, a subunit of flavocytochrome b558. Using NMR titration, we first identified the proline-rich region of p22phox that is essential for binding to the tandem SH3 domains of p47phox. We subsequently determined the solution structure of the p47phox tandem SH3 domains complexed with the proline-rich peptide of p22phox using NMR spectroscopy. In contrast to the intertwined dimer reported for the crystal state, the solution structure is a monomer. The central region of the p22phox peptide forms a polyproline type II helix that is sandwiched by the N- and C-terminal SH3 domains, as was observed in the crystal structure, whereas the C-terminal region of the peptide takes on a short alpha-helical conformation that provides an additional binding site with the N-terminal SH3 domain. Thus, the C-terminal alpha-helical region of the p22phox peptide increases the binding affinity for the tandem SH3 domains of p47phox more than 10-fold.

NMR solution structure of the tandem Src homology 3 domains of p47phox complexed with a p22phox-derived proline-rich peptide.,Ogura K, Nobuhisa I, Yuzawa S, Takeya R, Torikai S, Saikawa K, Sumimoto H, Inagaki F J Biol Chem. 2006 Feb 10;281(6):3660-8. Epub 2005 Dec 2. PMID:16326715<ref>PMID:16326715</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 1wlp" style="background-color:#fffaf0;"></div>

==See Also==
*[[Flavocytochrome 3D structures|Flavocytochrome 3D structures]]
*[[NADPH oxidase 3D structures|NADPH oxidase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Inagaki F]]
[[Category: Ogura K]]
[[Category: Saikawa K]]
[[Category: Sumimoto H]]
[[Category: Torikai S]]
[[Category: Yuzawa S]]