View source for 1zhq


==Crystal structure of apo MVL==
<StructureSection load='1zhq' size='340' side='right'caption='[[1zhq]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1zhq]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Microcystis_viridis Microcystis viridis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZHQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ZHQ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1zhq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zhq OCA], [https://pdbe.org/1zhq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1zhq RCSB], [https://www.ebi.ac.uk/pdbsum/1zhq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1zhq ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/MVL_MICVR MVL_MICVR] Carbohydrate-binding protein that binds oligomannosides such as Man(6)GlcNAc(2) with sub-micromolar affinities. The specificity of MVL is unique in that its minimal target comprises the Man-alpha-(1->6)-Man-beta-(1->4)-GlcNAc-beta-(1->4)-GlcNAc tetrasaccharide core (Man(2)A) found in N-linked oligomannosides. Displays hemagglutininating activity on rabbit, horse and hen erythrocytes. This activity is inhibited by yeast mannan. Does not bind mono- and disachharides. Inhibits HIV-1 envelope-mediated cell fusion at nanomolar concentrations through carbohydrate-mediated interactions with high-mannose residues on the surface of the HIV envelope glycoprotein gp120.  Unexpectedly for a lectin, one of the 2 oligomannose binding sites of MVL can catalyze the cleavage of chitin fragments (such as chitotriose, i.e. GlcNAc(3) or GlcNAc-beta-(1->4)-GlcNAcbeta-(1->4)-GlcNAc, and chitotetraose, i.e. GlcNAc(4)) to GlcNAc. This weak glycosidase activity is restricted to the C-terminal carbohydrate-binding site. Does not cleave Man(3)GlcNAc(2) or the tetrasaccharide Man(2)A.

==See Also==
*[[Mannose-binding protein|Mannose-binding protein]]
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Microcystis viridis]]
[[Category: Bewley CA]]
[[Category: Cai M]]
[[Category: Clore GM]]
[[Category: Lee JY]]
[[Category: Williams DC]]