View source for 1zug


==STRUCTURE OF PHAGE 434 CRO PROTEIN, NMR, 20 STRUCTURES==
<StructureSection load='1zug' size='340' side='right'caption='[[1zug]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1zug]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Phage_434 Phage 434]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZUG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ZUG FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1zug FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zug OCA], [https://pdbe.org/1zug PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1zug RCSB], [https://www.ebi.ac.uk/pdbsum/1zug PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1zug ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/RCRO_BP434 RCRO_BP434] Cro represses genes normally expressed in early phage development and is necessary for the late stage of lytic growth. It does this by binding to the OL and OR operators regions normally used by the repressor protein for lysogenic maintenance.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zu/1zug_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1zug ConSurf].
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== Publication Abstract from PubMed ==
1H NMR resonances of the phage 434 Cro protein were assigned using standard 2D NMR methods, and its solution structure determined using 867 distance constraints in distance geometry (DIANA) calculations ultimately refined by restrained molecular dynamics (GROMOS). In the 20 best NMR structures, the average pairwise backbone and heavy atom RMSDs are 0.63 +/- 0.14 and 1.53 +/- 0.15 A, respectively, for the structurally well-defined residues 4-65. Residues 1-3 and 66-71 at the N- and C-termini are structurally disordered. The region 4-65 includes five alpha-helices and tight turns which define the hydrophobic core of the protein. The backbone and heavy atom RMSDs for residues 4-65 are 0.92 +/- 0.12 and 1.99 +/- 0.12 A, respectively, for the NMR versus the crystal structures, but there are significant differences in the side-chain conformations and solvent accessibilities for some core residues. Analytical ultracentrifugation experiments confirm that 434 Cro is monomeric even at the high NMR concentrations. 434 Cro folding under NMR solution conditions is two-state as indicated by coincident urea denaturation curves from circular dichroism and intrinsic fluorescence measurements. They yield values for 434 Cro stability which show good correspondence to the free energy for global unfolding determined by NMR hydrogen exchange measurements for the slowest exchanging amide protons.

Three-dimensional solution structure and stability of phage 434 Cro protein.,Padmanabhan S, Jimenez MA, Gonzalez C, Sanz JM, Gimenez-Gallego G, Rico M Biochemistry. 1997 May 27;36(21):6424-36. PMID:9174359<ref>PMID:9174359</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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== References ==
<references/>
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</StructureSection>
[[Category: Large Structures]]
[[Category: Phage 434]]
[[Category: Gimenez-Gallego G]]
[[Category: Gonzalez C]]
[[Category: Jimenez MA]]
[[Category: Padmanabhan S]]
[[Category: Rico M]]
[[Category: Sanz JM]]