View source for 21dh


==Crystal structure of MBP-fused Monobody P' in complex with HPPU==
<StructureSection load='21dh' size='340' side='right'caption='[[21dh]], [[Resolution|resolution]] 2.57&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[21dh]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=21DH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=21DH FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.57&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A1MC1:1-(4-hydroxyphenyl)-3-phenyl-urea'>A1MC1</scene>, <scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=PRD_900018:beta-maltose'>PRD_900018</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=21dh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=21dh OCA], [https://pdbe.org/21dh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=21dh RCSB], [https://www.ebi.ac.uk/pdbsum/21dh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=21dh ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/MALE_ECOLI MALE_ECOLI] Involved in the high-affinity maltose membrane transport system MalEFGK. Initial receptor for the active transport of and chemotaxis toward maltooligosaccharides.
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== Publication Abstract from PubMed ==
Monobodies, engineered protein scaffolds derived from the fibronectin type III domain, are powerful alternatives to conventional antibodies. While the native scaffold is robust, engineering the variable loops can often compromise solubility and promote aggregation. Here, we report the crystallization and structure determination at 2.57 A resolution of a monobody (Mb-P') engineered to bind the synthetic small molecule HPPU [1-(4-hydroxyphenyl)-3-phenylurea] with nanomolar affinity. Although Mb-P' exhibited severe polydispersity and heterogeneous oligomerization in solution, N-terminal fusion with maltose-binding protein (MBP) using an optimized linker successfully yielded monodisperse species and diffraction-quality crystals. The crystal structure exhibited pseudo-D(3) symmetry in the asymmetric unit, in which the MBP moiety interacts with and partially covers the F and G beta-strands of the monobody. This steric masking suggests that MBP acts as a solubility enhancer by shielding the aggregation-prone surface patches generated by loop engineering. Our results demonstrate that this fusion strategy effectively enables structural studies of aggregation-prone proteins obtained from engineered scaffolds.

Crystallization and X-ray structure of a highly aggregation-prone monobody engineered for high-affinity small-molecule recognition.,Endo K, Umemoto S, Tsuzuki N, Okumura H, Sato Y, Yoshii T, Tsukiji S, Nagano S, Murakami H, Hino T Acta Crystallogr F Struct Biol Commun. 2026 Mar 1;82(Pt 3):75-82. doi: , 10.1107/S2053230X26000798. Epub 2026 Feb 9. PMID:41660693<ref>PMID:41660693</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 21dh" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Escherichia coli K-12]]
[[Category: Large Structures]]
[[Category: Synthetic construct]]
[[Category: Endo K]]
[[Category: Hino T]]
[[Category: Murakami H]]
[[Category: Nagano S]]
[[Category: Okumura H]]
[[Category: Sato Y]]
[[Category: Tsukiji S]]
[[Category: Umemoto S]]