View source for 21ji


==Crystal structure of isoprimeverose-producing enzyme from Phaeoacremonium minimum==
<StructureSection load='21ji' size='340' side='right'caption='[[21ji]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[21ji]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Phaeoacremonium_minimum Phaeoacremonium minimum] and [https://en.wikipedia.org/wiki/Rattus_rattus Rattus rattus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=21JI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=21JI FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=P6G:HEXAETHYLENE+GLYCOL'>P6G</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=21ji FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=21ji OCA], [https://pdbe.org/21ji PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=21ji RCSB], [https://www.ebi.ac.uk/pdbsum/21ji PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=21ji ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
Isoprimeverose-producing oligoxyloglucan hydrolases (IPases), which belong to glycoside hydrolase family 3 (GH3), release isoprimeverose (alpha-d-xylopyranosyl-(1--&gt;6)-d-glucopyranose) from the non-reducing end of xyloglucan oligosaccharides. The IPase from Phaeoacremonium minimum (PmIPase) exhibits distinctive features compared to its homologous IPase in Aspergillus oryzae, IpeA. The hydrolytic activity of A. oryzae IpeA was inhibited by galactosylation of the second xylopyranosyl residue at the nonreducing end of the substrate, whereas PmIPase was unaffected. To elucidate the structural basis of the unique features of PmIPase, its crystal structure was determined. PmIPase forms a homo-dimer, and its active site is formed by two monomers. Structural comparison with IpeA revealed that the active-site structures are highly conserved between both enzymes, but with some differences, including Phe172 at subsite -1 and Trp624 at a positive subsite in PmIPase (Met173 and Ile625 in IpeA, respectively). Trp624 is from the adjacent monomer and seems to be located at the putative binding position of the galactose residue at subsite +1. When the substrate was a mixture of XXXG, XXLG/XLXG, and XLLG, the W624I mutant showed lower specificity for XLLG than XXXG and XXLG/XLXG, although the wild-type enzyme degraded all three substrates with equal efficiency. This result indicates that Trp624 in PmIPase is involved in the recognition of the galactose residue at subsite +1. These findings provide structural insights into the characteristics and diversity of IPases that adapt to the side chain structures of xyloglucan.

Structural basis for galactose side-chain recognition in isoprimeverose-producing oligoxyloglucan hydrolase from Phaeoacremonium minimum.,Nakamichi Y, Watanabe M, Yaoi K, Matsuzawa T Int J Biol Macromol. 2026 Mar 26;357:151665. doi: 10.1016/j.ijbiomac.2026.151665. PMID:41903633<ref>PMID:41903633</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 21ji" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Large Structures]]
[[Category: Phaeoacremonium minimum]]
[[Category: Rattus rattus]]
[[Category: Matsuzawa T]]
[[Category: Nakamichi Y]]
[[Category: Watanabe M]]
[[Category: Yaoi K]]