View source for 26wc


==Local refinement of the mpox virus A35R protein in complexed with 17H1 Fab==
<StructureSection load='26wc' size='340' side='right'caption='[[26wc]], [[Resolution|resolution]] 3.63&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[26wc]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Monkeypox_virus Monkeypox virus] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=26WC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=26WC FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.63&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=26wc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=26wc OCA], [https://pdbe.org/26wc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=26wc RCSB], [https://www.ebi.ac.uk/pdbsum/26wc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=26wc ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/PG161_MONPV PG161_MONPV] Forms a complex with OPG162 and OPG190 to coordinate the incorporation of OPG164 into wrapped enveloped virion (EV) membranes and, subsequently, the production of actin tails. Therefore plays an essential role in efficient cell-to-cell spread of viral particles.[UniProtKB:P68617]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The recent resurgence of the mpox virus (MPXV) has raised global health concerns due to its potential to cause severe illness in vulnerable populations. Targeting the extracellular enveloped virus (EEV) protein A35R is a promising strategy to restrict viral dissemination within the host. In this study, we combined mRNA-LNP immunization with the Beacon Optofluidic system to rapidly screen and isolate high-affinity monoclonal antibodies. One of these antibodies, 17H1, exhibited exceptional neutralization against authentic MPXV. Cryo-electron microscopy (Cryo-EM) analysis revealed that 17H1 binds to a specific epitope at the distal ends of the A35R dimer. The interaction is stabilized by a unique network of hydrogen bonds and salt bridges, particularly involving residue E120, distinguishing its binding mode from previously reported A35R antibodies. Furthermore, 17H1 exhibited complete protective efficacy against MPXV infection in vivo and significantly reduced pulmonary viral load and lung pathogenesis. These findings highlight 17H1 as a promising therapeutic candidate for novel mpox interventions.

Discovery and Structural Characterization of a Highly Protective Neutralizing Antibody Targeting the Mpox Virus A35R Protein.,Bai S, Song S, Wang X, Xiao Y, Long Y, Wu F, Wang F, Fan Z, Xu J, He M, Lu H MedComm (2020). 2026 Jun 23;7(7):e70804. doi: 10.1002/mco2.70804. eCollection , 2026 Jul. PMID:42358411<ref>PMID:42358411</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 26wc" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Monkeypox virus]]
[[Category: Mus musculus]]
[[Category: He MZ]]
[[Category: Xiao YX]]