View source for 27gz


==Juvenile hormone binding protein complexed with 2-Methyl-1-(4-butylbenzyl)-benzimidazole==
<StructureSection load='27gz' size='340' side='right'caption='[[27gz]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[27gz]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bombyx_mori Bombyx mori]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=27GZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=27GZ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A1MHI:1-[(4-butylphenyl)methyl]-2-methyl-benzimidazole'>A1MHI</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=27gz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=27gz OCA], [https://pdbe.org/27gz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=27gz RCSB], [https://www.ebi.ac.uk/pdbsum/27gz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=27gz ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q9U556_BOMMO Q9U556_BOMMO] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The authors previously reported that 2-methylbenzimidazole derivatives (MBIs) exhibit insect growth-regulating activity against the silkworm, Bombyx mori. However, despite their unique effects on juvenile hormone (JH)-related endocrine pathways, the precise mode of action of MBIs remained unclear. In the present study, the interactions between MBIs and the lepidopteran hemolymph JH-binding protein (JHBP), a key regulator of JH transport and activity, were investigated using multiple approaches, including in vitro binding affinity assays, X-ray crystallography, and molecular docking simulations. A series of MBIs bearing a 1-(4-alkoxybenzyl) group, which exhibited potent insect growth-regulating activity, showed high binding affinity and structural compatibility with the JH-binding pocket of JHBP. In contrast, 1-(4-alkylbenzyl) MBIs, which displayed weak or negligible insect growth-regulating activity, exhibited low affinity for JHBP. These findings suggest that the insect growth-regulating activity of MBIs is mediated through inhibition of JHBP function, likely by disrupting the precise regulation of JH concentration in the hemolymph during larval development and pupal metamorphosis.

Structural Characteristics for the Interaction of 1-Benzyl-2-Methylbenzimidazoles as Insect Growth Regulators and Juvenile Hormone Binding Protein.,Gunasekara UKDSS, Inoue K, Fujimoto Z, Henmi S, Tsuchiya W, Suzuki R, Kutsuwada K, Ikeda I, Yamazaki T, Shiotsuki T Insects. 2026 Jun 22;17(6):657. doi: 10.3390/insects17060657. PMID:42355390<ref>PMID:42355390</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 27gz" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Bombyx mori]]
[[Category: Large Structures]]
[[Category: Fujimoto Z]]
[[Category: Shiotsuki T]]
[[Category: Suzuki R]]
[[Category: Tsuchiya W]]