View source for 2chk


==NMR structure of TLLLLT quadruplex==
<StructureSection load='2chk' size='340' side='right'caption='[[2chk]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2chk]] is a 4 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CHK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CHK FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LCG:[(1R,3R,4R,7S)-7-HYDROXY-3-(GUANIN-9-YL)-2,5-DIOXABICYCLO[2.2.1]HEPT-1-YL]METHYL+DIHYDROGEN+PHOSPHATE'>LCG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2chk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2chk OCA], [https://pdbe.org/2chk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2chk RCSB], [https://www.ebi.ac.uk/pdbsum/2chk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2chk ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
We have determined the NMR solution structures of the quadruplexes formed by d(TGLGLT) and d(TL4T), where L denotes LNA (locked nucleic acid) modified G-residues. Both structures are tetrameric, parallel and right-handed and the native global fold of the corresponding DNA quadruplex is retained upon introduction of the LNA nucleotides. However, local structural alterations are observed owing to the locked LNA sugars. In particular, a distinct change in the sugar-phosphate backbone is observed at the G2pL3 and L2pL3 base steps and sequence dependent changes in the twist between tetrads are also seen. Both the LNA modified quadruplexes have raised thermostability as compared to the DNA quadruplex. The quadruplex-forming capability of d(TGLGLT) is of particular interest as it expands the design flexibility for stable parallel LNA quadruplexes and shows that LNA nucleotides can be mixed with DNA or other modified nucleic acids. As such, LNA-based quadruplexes can be decorated by a variety of chemical modifications. Such LNA quadruplex scaffolds might find applications in the developing field of nanobiotechnology.

NMR solution structures of LNA (locked nucleic acid) modified quadruplexes.,Nielsen JT, Arar K, Petersen M Nucleic Acids Res. 2006 Apr 13;34(7):2006-14. Print 2006. PMID:16614450<ref>PMID:16614450</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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== References ==
<references/>
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</StructureSection>
[[Category: Large Structures]]
[[Category: Nielsen JT]]
[[Category: Petersen M]]