View source for 2ett


==Solution Structure of Human Sorting Nexin 22 PX Domain==
<StructureSection load='2ett' size='340' side='right'caption='[[2ett]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2ett]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ETT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ETT FirstGlance]. <br>
</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SNX22 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ett FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ett OCA], [https://pdbe.org/2ett PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ett RCSB], [https://www.ebi.ac.uk/pdbsum/2ett PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ett ProSAT], [https://www.topsan.org/Proteins/CESG/2ett TOPSAN]</span></td></tr>
</table>
== Function ==
[[https://www.uniprot.org/uniprot/SNX22_HUMAN SNX22_HUMAN]] May be involved in several stages of intracellular trafficking (By similarity). Interacts with membranes containing phosphatidylinositol 3-phosphate (PtdIns(3P)). 
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/et/2ett_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ett ConSurf].
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== Publication Abstract from PubMed ==
The sorting nexins (SNXs) constitute a large group of PX domain-containing proteins that play critical roles in protein trafficking. We report here the solution structure of human sorting nexin 22 (SNX22). Although SNX22 has &lt;30% sequence identity with any PX domain protein of known structure, it was found to contain the alpha/beta fold and compact structural core characteristic of PX domains. Analysis of the backbone dynamics of SNX22 by NMR relaxation measurements revealed that the two walls of the ligand binding cleft undergo internal motions: on the picosecond timescale for the beta1/beta2 loop and on the micro- to millisecond timescale for the loop between the polyproline motif and helix alpha2. Regions of the SNX22 structure that differ from those of other PX domains include the loop connecting strands beta1 and beta2 and the loop connecting helices alpha1 and alpha2, which appear to be more mobile than corresponding loops in other known structures. The interaction of dibutanoyl-phosphatidylinositol-3-phosphate (dibutanoyl-PtdIns(3)P) with SNX22 was investigated by an NMR titration experiment, which identified the binding site in a basic cleft and indicated that ligand binding leads only to a local structural rearrangement as has been found with other PX domains. Because motions in the loops are damped out when dibutanoyl-PtdIns(3)P binds, entropic effects could contribute to the lower affinity of SNX22 for this ligand compared to other PX domains.

Solution structure of human sorting nexin 22.,Song J, Zhao KQ, Newman CL, Vinarov DA, Markley JL Protein Sci. 2007 May;16(5):807-14. Epub 2007 Mar 30. PMID:17400918<ref>PMID:17400918</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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==See Also==
*[[Sorting nexin 3D structures|Sorting nexin 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Human]]
[[Category: Large Structures]]
[[Category: Structural genomic]]
[[Category: Lee, M S]]
[[Category: Markley, J L]]
[[Category: Newman, C L]]
[[Category: Song, J]]
[[Category: Tyler, R C]]
[[Category: Zhao, Q]]
[[Category: Bc019655]]
[[Category: Cesg]]
[[Category: Hs 157607]]
[[Category: PSI, Protein structure initiative]]
[[Category: Protein transport]]
[[Category: Px domain]]
[[Category: Snx22_human]]
[[Category: Sorting nexin 22]]