View source for 2hua


==Solution Structure of CSFV IRES Domain IIa==
<StructureSection load='2hua' size='340' side='right'caption='[[2hua]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2hua]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HUA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HUA FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2hua FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hua OCA], [https://pdbe.org/2hua PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2hua RCSB], [https://www.ebi.ac.uk/pdbsum/2hua PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2hua ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Internal ribosome entry site (IRES) RNAs from the hepatitis C virus (HCV) and classical swine fever virus (CSFV) coordinate cap-independent assembly of eukaryotic 48S initiation complexes, consisting of the 40S ribosomal subunit, eukaryotic initiation factor (eIF) 3 and the eIF2/GTP/Met-tRNA(i)(Met) ternary complex. Here, we report that these IRESes also play a functional role during 80S ribosome assembly downstream of 48S complex formation, in promoting eIF5-induced GTP hydrolysis and eIF2/GDP release from the initiation complex. We show that this function is encoded in their independently folded IRES domain II and that it depends both on its characteristic bent conformation and two conserved RNA motifs, an apical hairpin loop and a loop E. Our data suggest a general mode of subunit joining in HCV and HCV-like IRESes.

HCV and CSFV IRES domain II mediate eIF2 release during 80S ribosome assembly.,Locker N, Easton LE, Lukavsky PJ EMBO J. 2007 Feb 7;26(3):795-805. Epub 2007 Jan 25. PMID:17255934<ref>PMID:17255934</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2hua" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Easton LE]]
[[Category: Locker N]]
[[Category: Lukavsky PJ]]