View source for 2ij0


==Structural basis of T cell specificity and activation by the bacterial superantigen toxic shock syndrome toxin-1==
<StructureSection load='2ij0' size='340' side='right'caption='[[2ij0]], [[Resolution|resolution]] 2.25&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2ij0]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus aureus" (rosenbach 1884) zopf 1885] and [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IJ0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2IJ0 FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ij0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ij0 OCA], [https://pdbe.org/2ij0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ij0 RCSB], [https://www.ebi.ac.uk/pdbsum/2ij0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ij0 ProSAT]</span></td></tr>
</table>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ij/2ij0_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ij0 ConSurf].
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== Publication Abstract from PubMed ==
Superantigens (SAGs) bind simultaneously to major histocompatibility complex (MHC) and T-cell receptor (TCR) molecules, resulting in the massive release of inflammatory cytokines that can lead to toxic shock syndrome (TSS) and death. A major causative agent of TSS is toxic shock syndrome toxin-1 (TSST-1), which is unique relative to other bacterial SAGs owing to its structural divergence and its stringent TCR specificity. Here, we report the crystal structure of TSST-1 in complex with an affinity-matured variant of its wild-type TCR ligand, human T-cell receptor beta chain variable domain 2.1. From this structure and a model of the wild-type complex, we show that TSST-1 engages TCR ligands in a markedly different way than do other SAGs. We provide a structural basis for the high TCR specificity of TSST-1 and present a model of the TSST-1-dependent MHC-SAG-TCR T-cell signaling complex that is structurally and energetically unique relative to those formed by other SAGs. Our data also suggest that protein plasticity plays an exceptionally significant role in this affinity maturation process that results in more than a 3000-fold increase in affinity.

Structural basis of T-cell specificity and activation by the bacterial superantigen TSST-1.,Moza B, Varma AK, Buonpane RA, Zhu P, Herfst CA, Nicholson MJ, Wilbuer AK, Seth NP, Wucherpfennig KW, McCormick JK, Kranz DM, Sundberg EJ EMBO J. 2007 Feb 21;26(4):1187-97. Epub 2007 Feb 1. PMID:17268555<ref>PMID:17268555</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 2ij0" style="background-color:#fffaf0;"></div>

==See Also==
*[[T-cell receptor 3D structures|T-cell receptor 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Human]]
[[Category: Large Structures]]
[[Category: Buonpane, R A]]
[[Category: Herfst, C A]]
[[Category: Kranz, D M]]
[[Category: McCormick, J K]]
[[Category: Moza, B]]
[[Category: Nicholson, M J]]
[[Category: Nulifer, S]]
[[Category: Sundberg, E J]]
[[Category: Varma, A K]]
[[Category: Wilbuer, A K]]
[[Category: Wucherpfenning, K W]]
[[Category: Zhu, P]]
[[Category: Protein binding]]
[[Category: Protein-protein interaction]]
[[Category: Superantigen]]