View source for 2jes


==Portal protein (gp6) from bacteriophage SPP1==
<StructureSection load='2jes' size='340' side='right'caption='[[2jes]], [[Resolution|resolution]] 3.40&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2jes]] is a 26 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_phage_SPP1 Bacillus phage SPP1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JES OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JES FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.4&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2jes FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jes OCA], [https://pdbe.org/2jes PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2jes RCSB], [https://www.ebi.ac.uk/pdbsum/2jes PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2jes ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/PORTL_BPSPP PORTL_BPSPP] Forms the portal vertex of the capsid (PubMed:17363899). This portal plays critical roles in head assembly, genome packaging, neck/tail attachment, and genome ejection. The portal protein multimerizes as a single ring-shaped homododecamer arranged around a central channel. Binds to the terminase subunits to form the packaging machine.<ref>PMID:17363899</ref> 
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/je/2jes_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2jes ConSurf].
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== Publication Abstract from PubMed ==
Tailed bacteriophages and herpesviruses load their capsids with DNA through a tunnel formed by the portal protein assembly. Here we describe the X-ray structure of the bacteriophage SPP1 portal protein in its isolated 13-subunit form and the pseudoatomic structure of a 12-subunit assembly. The first defines the DNA-interacting segments (tunnel loops) that pack tightly against each other forming the most constricted part of the tunnel; the second shows that the functional dodecameric state must induce variability in the loop positions. Structural observations together with geometrical constraints dictate that in the portal-DNA complex, the loops form an undulating belt that fits and tightly embraces the helical DNA, suggesting that DNA translocation is accompanied by a 'mexican wave' of positional and conformational changes propagating sequentially along this belt.

Structural framework for DNA translocation via the viral portal protein.,Lebedev AA, Krause MH, Isidro AL, Vagin AA, Orlova EV, Turner J, Dodson EJ, Tavares P, Antson AA EMBO J. 2007 Apr 4;26(7):1984-94. Epub 2007 Mar 15. PMID:17363899<ref>PMID:17363899</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 2jes" style="background-color:#fffaf0;"></div>

==See Also==
*[[Portal protein|Portal protein]]
*[[Portal protein 3D structures|Portal protein 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Bacillus phage SPP1]]
[[Category: Large Structures]]
[[Category: Antson AA]]
[[Category: Dodson EJ]]
[[Category: Isidro AL]]
[[Category: Krause MH]]
[[Category: Lebedev AA]]
[[Category: Orlova EV]]
[[Category: Tavares P]]
[[Category: Turner J]]
[[Category: Vagin AA]]