View source for 2jml


==Solution structure of the N-terminal domain of CarA repressor==
<StructureSection load='2jml' size='340' side='right'caption='[[2jml]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2jml]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Myxococcus_xanthus_DK_1622 Myxococcus xanthus DK 1622]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JML OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JML FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2jml FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jml OCA], [https://pdbe.org/2jml PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2jml RCSB], [https://www.ebi.ac.uk/pdbsum/2jml PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2jml ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q1DDV9_MYXXD Q1DDV9_MYXXD] 
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jm/2jml_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2jml ConSurf].
<div style="clear:both"></div>
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== Publication Abstract from PubMed ==
Blue light induces carotenogenesis in Myxococcus xanthus. The carB operon encodes all but one of the structural genes involved, and its expression is regulated by the CarA-CarS repressor-antirepressor pair. In the dark, CarA-operator binding represses carB. CarS, produced on illumination, interacts physically with CarA to dismantle the CarA-operator complex and activate carB. Both operator and CarS bind to the autonomously folded N-terminal domain of CarA, CarA(Nter), which in excess represses carB. Here, we report the NMR structure of CarA(Nter), and map residues that interact with operator and CarS by NMR chemical shift perturbations, and in vivo and in vitro analyses of site-directed mutants. We show CarA(Nter) adopts the winged-helix topology of MerR-family DNA-binding domains, and conserves the majority of the helix-turn-helix and wing contacts with DNA. Tellingly, helix alpha2 in CarA, a key element in operator DNA recognition, is also critical for interaction with CarS, implying that the CarA-CarS protein-protein and the CarA-operator protein-DNA interfaces overlap. Thus, binding of CarA to operator and to antirepressor are mutually exclusive, and CarA may discern structural features in the acidic CarS protein that resemble operator DNA. Repressor inactivation by occluding the DNA-binding region may be a recurrent mechanism of action for acidic antirepressors.

Structural basis for operator and antirepressor recognition by Myxococcus xanthus CarA repressor.,Navarro-Aviles G, Jimenez MA, Perez-Marin MC, Gonzalez C, Rico M, Murillo FJ, Elias-Arnanz M, Padmanabhan S Mol Microbiol. 2007 Feb;63(4):980-94. PMID:17233828<ref>PMID:17233828</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 2jml" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Myxococcus xanthus DK 1622]]
[[Category: Elias-Arnanz M]]
[[Category: Gonzalez C]]
[[Category: Jimenez M]]
[[Category: Murillo FJ]]
[[Category: Padmanabhan S]]
[[Category: Perez-Marin MC]]
[[Category: Rico M]]