View source for 2jmr


==NMR structure of the E. coli type 1 pilus subunit FimF==
<StructureSection load='2jmr' size='340' side='right'caption='[[2jmr]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2jmr]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JMR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JMR FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 20 models</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2jmr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jmr OCA], [https://pdbe.org/2jmr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2jmr RCSB], [https://www.ebi.ac.uk/pdbsum/2jmr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2jmr ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/FIMF_ECOLI FIMF_ECOLI] Involved in regulation of length and mediation of adhesion of type 1 fimbriae (but not necessary for the production of fimbriae). Involved in the integration of FimH in the fimbriae.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jm/2jmr_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2jmr ConSurf].
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== Publication Abstract from PubMed ==
Type 1 pili from uropathogenic Escherichia coli strains mediate bacterial attachment to target receptors on the host tissue. They are composed of up to 3000 copies of the subunit FimA, which form the stiff, helical pilus rod, and the subunits FimF, FimG, and FimH, which form the linear tip fibrillum. All subunits in the pilus interact via donor strand complementation, in which the incomplete immunoglobulin-like fold of each subunit is complemented by insertion of an N-terminal extension from the following subunit. We determined the NMR structure of a monomeric, self-complemented variant of FimF, FimF(F), which has a second FimF donor strand segment fused to its C-terminus that enables intramolecular complementation of the FimF fold. NMR studies on bimolecular complexes between FimF(F) and donor strand-depleted variants of FimF and FimG revealed that the relative orientations of neighboring domains in the tip fibrillum cover a wide range. The data provide strong support for the intrinsic flexibility of the tip fibrillum. They lend further support to the hypothesis that this flexibility would significantly increase the probability that the adhesin at the distal end of the fibrillum successfully targets host cell receptors.

NMR structure of the Escherichia coli type 1 pilus subunit FimF and its interactions with other pilus subunits.,Gossert AD, Bettendorff P, Puorger C, Vetsch M, Herrmann T, Glockshuber R, Wuthrich K J Mol Biol. 2008 Jan 18;375(3):752-63. Epub 2007 Nov 1. PMID:18048056<ref>PMID:18048056</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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== References ==
<references/>
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Large Structures]]
[[Category: Bettendorff P]]
[[Category: Fiorito F]]
[[Category: Glockshuber R]]
[[Category: Gossert AD]]
[[Category: Herrmann T]]
[[Category: Hiller S]]
[[Category: Puorger C]]
[[Category: Vetsch M]]
[[Category: Wuthrich K]]