View source for 2jnv


==Solution structure of C-terminal domain of NifU-like protein from Oryza sativa==
<StructureSection load='2jnv' size='340' side='right'caption='[[2jnv]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2jnv]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Oryza_sativa Oryza sativa]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JNV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JNV FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2jnv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jnv OCA], [https://pdbe.org/2jnv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2jnv RCSB], [https://www.ebi.ac.uk/pdbsum/2jnv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2jnv ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/NIFU1_ORYSJ NIFU1_ORYSJ] Molecular scaffold for [Fe-S] cluster assembly of chloroplastic iron-sulfur proteins (By similarity).
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jn/2jnv_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2jnv ConSurf].
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== Publication Abstract from PubMed ==
OsCnfU-1A is a chloroplast-type Nfu-like protein that consists of tandem repeats sharing high sequence homology. Domain I of this protein, but not domain II, has a C-X-X-C motif that is thought to assemble an iron-sulphur cluster. Herein we report the solution structure of OsCnfU-1A domain I (73-153). Although OsCnfU-1A domain I is structurally similar to OsCnfU-1A domain II (154-226), the electrostatic surface potential of the 2 domains differs. Domain I has an acidic surface, whereas that of domain II is predominantly basic. Chemical shift perturbation studies on OsCnfU-1A domain I and domain II with ferredoxin revealed negligible chemical shift changes in domain I, whereas much larger chemical shift changes were observed in domain II. The residues with larger chemical shift changes were located on the basic surface of domain II. Considering that ferredoxin is predominantly negatively charged, we propose the following hypothesis: First, an iron-sulphur cluster is assembled on domain I. Next, domain II interacts with the ferredoxin, thus tethering domain I close to the ferredoxin. Finally, domain I transfers the iron-sulphur cluster to the ferredoxin. Thus, domain II facilitates the efficient transfer of the iron-sulphur cluster from domain I to the ferredoxin.

The cooperative role of OsCnfU-1A domain I and domain II in the iron sulphur cluster transfer process as revealed by NMR.,Saio T, Kumeta H, Ogura K, Yokochi M, Asayama M, Katoh S, Katoh E, Teshima K, Inagaki F J Biochem. 2007 Jul;142(1):113-21. Epub 2007 Jun 1. PMID:17545250<ref>PMID:17545250</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 2jnv" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Oryza sativa]]
[[Category: Inagaki F]]
[[Category: Katoh E]]
[[Category: Katoh S]]
[[Category: Kumeta H]]
[[Category: Ogura K]]
[[Category: Saio T]]
[[Category: Yokochi M]]