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==Solution structure of a let-7 miRNA:lin-41 mRNA complex from C. elegans==
<StructureSection load='2jxv' size='340' side='right'caption='[[2jxv]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2jxv]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JXV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JXV FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2jxv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jxv OCA], [https://pdbe.org/2jxv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2jxv RCSB], [https://www.ebi.ac.uk/pdbsum/2jxv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2jxv ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Let-7 microRNA (miRNA) regulates heterochronic genes in developmental timing of the nematode Caenorhabditis elegans. Binding of miRNA to messenger RNA (mRNA) and structural features of the complex are crucial for gene silencing. We herein present the NMR solution structure of a model mimicking the interaction of let-7 miRNA with its complementary site (LCS 2) in the 3' untranslated region (3'-UTR) of the lin-41 mRNA. A structural study was performed by NMR spectroscopy using NOE restraints, torsion angle restraints and residual dipolar couplings. The 33-nt RNA construct folds into a stem-loop structure that features two stem regions which are separated by an asymmetric internal loop. One of the stems comprises a GU wobble base pair, which does not alter its overall A-form RNA conformation. The asymmetric internal loop adopts a single, well-defined structure in which three uracils form a base triple, while two adenines form a base pair. The 3D structure of the construct gives insight into the structural aspects of interactions between let-7 miRNA and lin-41 mRNA.

Solution structure of a let-7 miRNA:lin-41 mRNA complex from C. elegans.,Cevec M, Thibaudeau C, Plavec J Nucleic Acids Res. 2008 Apr;36(7):2330-7. Epub 2008 Feb 22. PMID:18296482<ref>PMID:18296482</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2jxv" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Large Structures]]
[[Category: Cevec M]]
[[Category: Plavec J]]
[[Category: Thibaudeau C]]