View source for 2kcj


==solution structure of FAPP1 PH domain==
<StructureSection load='2kcj' size='340' side='right'caption='[[2kcj]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2kcj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KCJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KCJ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kcj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kcj OCA], [https://pdbe.org/2kcj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kcj RCSB], [https://www.ebi.ac.uk/pdbsum/2kcj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kcj ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/PKHA3_HUMAN PKHA3_HUMAN] Involved in Golgi to cell surface membrane traffic. Induces membrane tubulation. Binds preferentially to phosphatidylinositol 4-phosphate (PtdIns4P).<ref>PMID:15107860</ref> 
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kc/2kcj_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2kcj ConSurf].
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== Publication Abstract from PubMed ==
The mechanisms underlying Golgi targeting and vesiculation are unknown, although the responsible phosphatidylinositol 4-phosphate (PtdIns(4)P) ligand and four-phosphate-adaptor protein (FAPP) modules have been defined. The micelle-bound structure of the FAPP1 pleckstrin homology domain reveals how its prominent wedge independently tubulates Golgi membranes by leaflet penetration. Mutations compromising the exposed hydrophobicity of full-length FAPP2 abolish lipid monolayer binding and compression. The trafficking process begins with an electrostatic approach, phosphoinositide sampling and perpendicular penetration. Extensive protein contacts with PtdIns(4)P and neighbouring phospholipids reshape the bilayer and initiate tubulation through a conserved wedge with features shared by diverse protein modules.

Structural basis of wedging the Golgi membrane by FAPP pleckstrin homology domains.,Lenoir M, Coskun U, Grzybek M, Cao X, Buschhorn SB, James J, Simons K, Overduin M EMBO Rep. 2010 Apr;11(4):279-84. doi: 10.1038/embor.2010.28. Epub 2010 Mar 19. PMID:20300118<ref>PMID:20300118</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2kcj" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Coskun U]]
[[Category: James J]]
[[Category: Lenoir M]]
[[Category: Overduin M]]
[[Category: Simons K]]